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目的:阐明黄芩素在细胞培养中对流感病毒A/FM1/1/47(H1N1)的抑制机理。方法:通过结晶紫染色实验测定黄芩素对流感病毒的抑制作用,采用不同加药方式进一步研究黄芩素对病毒复制周期不同阶段的影响。通过RT-PCR方法比较黄芩素给药组和病毒模型组流感病毒M1基因mRNA的水平,确定黄芩素对病毒mRNA合成的抑制作用。结果:不同浓度的黄芩素在体外能够明显抑制流感病毒A/FM1/1/47(H1N1)和A/Beijing/32/92(H3N2)的感染,并呈剂量依赖。病毒感染后再加入药物黄芩素(治疗法)的抑制作用明显好于病毒感染前(预防法)和病毒感染同时(同时法)加入的抑制效应。在病毒感染6h、9h时,黄芩素给药组的病毒M1基因mRNA水平明显降低。结论:黄芩素体外可通过干扰流感病毒A/FM1/1/47(H1N1)中后期mRNA的合成抑制病毒的复制。
Objective: To elucidate the inhibitory mechanism of baicalein against influenza virus A / FM1 / 1/47 (H1N1) in cell culture. Methods: The inhibitory effect of baicalein on influenza virus was determined by crystal violet staining. The effects of baicalein on different stages of virus replication cycle were further studied by different dosing methods. The level of mRNA of M1 gene in the baicalein-administered group and the virus-treated group was compared by RT-PCR to determine the inhibitory effect of baicalein on the viral mRNA synthesis. Results: Different concentrations of baicalein significantly inhibited the infection of influenza A / FM1 / 1/47 (H1N1) and A / Beijing / 32/92 (H3N2) in a dose-dependent manner. The inhibitory effect of baicalein (treatment) added after virus infection was significantly better than that of virus infection (prophylaxis) and simultaneous viral infection (simultaneous treatment). At 6h and 9h after virus infection, the mRNA level of M1 gene in the baicalein-treated group was significantly decreased. Conclusion: Baicalein can inhibit virus replication in vitro by interfering with the mRNA synthesis in the late stage of influenza virus A / FM1 / 1/47 (H1N1).