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采用更加敏感的半重叠式T细胞受体（TCR）γ链特异性引物的多聚酶链反应（PCR）法,对28例急性淋巴细胞白血病（ALL）初治、完全缓解（CR）及骨髓移植（BMT）患儿的骨髓标本进行检测,用消化煮沸及酚抽提法分别对所有骨髓标本进行DNA提取,将PCR结果进行比较。结果表明,消化煮沸法用于微量标本的DNA提取优于酚抽提法。28例ALL患儿中16例检出TCRγ特异性条带,其中微小残留病（MRD）组18例;阳性检出12例,其中免疫分型标记为B细胞型的4例（25.0%）,免疫标记为T细胞型者全部出现TCRγ阳性条带。表明TCRγ基因重排并非克隆性T细胞增生所特有,部分ALL患儿存在双克隆重排。
Twenty-eight cases of primary lymphoblastic leukemia (ALL), complete remission (CR), and bone marrow transplantation using polymerase chain reaction (PCR) with more sensitive semi-overlapping T cell receptor (TCR) gamma chain specific primers ( The bone marrow samples of children with BMT) were detected, and all bone marrow samples were extracted by digestive boiling and phenol extraction. The PCR results were compared. The results showed that the digestion boiled method was superior to the phenol extraction method for the DNA extraction of trace samples. TCRγ-specific bands were detected in 16 of 28 children with ALL, of which 18 were in the minimal residual disease (MRD) group; 12 were positive in the MRD group, and 4 (25.0%) were in the B-cell immunophenotype. All patients with immunolabelled T cell types showed TCRγ positive bands. It was shown that TCRγ gene rearrangement is not unique to clonal T cell proliferation, and there are double clone rearrangements in some children with ALL.