论文部分内容阅读
目的研究短穗兔耳草对α-异硫氰酸萘酯(ANIT)所致肝损伤小鼠的保护作用。方法取昆明种小鼠60只,随机分为6组,每组10只,分别为正常组,模型组,联苯双酯组(0.15 g·kg~(-1)),短穗兔耳草提取物低、中、高剂量组(1.0,2.0,4.0 g·kg~(-1))。采用灌胃ANIT 100 mg·kg~(-1)花生油溶液的方法制备小鼠急性肝损伤模型,连续灌胃给药7 d,末次给药1 h后取血、取肝脏,检测血清中ALT、AST、TBA、TBIL和TNF-α的含量;检测肝组织匀浆中SOD、MDA、GSH-Px的含量,PCR检测肝组织中TNF-αm RNA的表达量,HE染色显微镜下观察肝组织病理变化。结果与模型组比较,短穗兔耳草低、中、高剂量组均能显著降低肝损伤小鼠血清ALT、AST、TBA、TBIL和TNF-α的水平和肝匀浆中MDA含量,同时显著升高小鼠肝匀浆中SOD和GSHPx的含量,差异均有统计学意义(P<0.05,P<0.01);短穗兔耳草提取物各剂量组均可显著下降肝组织中TNF-αm RNA的相对表达量(P<0.05,P<0.01)。病理切片显示短穗兔耳草各剂量组能显著改善肝组织的病理变化。结论短穗兔耳草对ANIT所致小鼠肝损伤有保护作用。
Objective To study the protective effect of Acridothera indica on hepatic injury induced by α-isothiocyanate (ANIT) in mice. Methods Sixty Kunming mice were randomly divided into 6 groups (10 rats in each group), which were normal group, model group, bifendate group (0.15 g · kg -1) Extracts of low, medium and high dose groups (1.0,2.0,4.0 g · kg -1). Acute liver injury model was established by intragastric administration of ANIT 100 mg · kg -1 peanut oil solution. The rats were administered intragastrically for 7 days. The rats were sacrificed at 1 h after the last administration of blood and the liver was taken for detection of ALT, AST, TBA, TBIL and TNF-α were measured. The contents of SOD, MDA and GSH-Px in liver homogenate were detected. The expression of TNF-αmRNA in liver tissues was detected by PCR. The pathological changes of liver tissue were observed under HE staining. . Results Compared with the model group, the levels of serum ALT, AST, TBA, TBIL and TNF-α and the content of MDA in liver homogenate of mice with low, medium and high dose of Erigeron breviscapus significantly decreased The content of SOD and GSHPx in liver homogenate increased significantly (P <0.05, P <0.01), and the content of TNF-αm in liver tissue of each group The relative expression of RNA (P <0.05, P <0.01). Pathological sections showed that each dose group of Pleurotus ostreatus could significantly improve the pathological changes of liver tissue. Conclusion Acridothera angustifolia has a protective effect on liver injury induced by ANIT in mice.