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Objective To study the effect of the amniotic membrane on enhancing the proliferation ofcorneal epithelia and YAC-1 cell. Methods After the primary culture of the rabbit’s corneal epithelia and YAC-1 cells, they were seeded on the upper surface or stromal matrix side of amniotic membrane respectively. The proliferation results were observed by MTT test. Results The amniotic membrane was found significantly enhancing the proliferation of corneal epithelia on the d1 ,d3 , and d5 after culture. The proliferation rate was 28.93% ,23.32% ,23.41 % (P<0 .05) respectively, but the d7 proliferation rate was 20.72% (P> 0.05). On the dl , d3 , d7 after culture , the YAC-1 cells proliferation rate was 34 .87% ,36 .28% ,33 .86% (P< 0.01) respectively. Conclusion Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly. Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surface, special atte
Objective To study the effect of the amniotic membrane on enhancing the proliferation of corneal epithelia and YAC-1 cell. Methods After the primary culture of the rabbit’s corneal epithelia and YAC-1 cells, they were seeded on the upper surface or stromal matrix side of amniotic The proliferation results were observed by MTT test. Results The amniotic membrane was found significantly reinforcing the proliferation of corneal epithelia on the d1, d3, and d5 after culture. The proliferation rate was 28.93%, 23.32%, 23.41% (P <0 .05) respectively, but the d7 proliferation rate was 20.72% (P> 0.05). On the dl, d3, d7 after culture, the YAC-1 cells proliferation rate was 34.87%, 36.28%, 33 .86% (P <0.01) respectively. Conclusion Our results demonstrated that the amniotic membrane could enhance the proliferation of both corneal epithelia and YAC-1 cells significantly. Although amniotic membrane has been suggested as an ideal material for reconstruction of ocular surgeries face, special atte