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目的研究p16蛋白在肝外胆管恶性肿瘤中表达的意义。方法用免疫组化ABC法 ,检测 41例肝外胆管癌和 12例胆管囊肿组织中 p16蛋白的表达情况。结果①p16蛋白在胆管囊肿组织的阳性表达率明显高于肝外胆管癌的阳性表达率 ,两者具有显著性差异。②p16蛋白的阳性表达率与肝外胆管癌的分化程度、临床分期 (Ⅰ～Ⅱ期与Ⅲ～Ⅳ期 )及是否有淋巴转移密切相关 (P <0 .0 5 ) ,而未发现 p16蛋白表达与胆管癌病理类型有相关性(P >0 .0 5 )。③p16蛋白表达阳性组生存时间明显长于 p16蛋白表达阴性组。 结论 p16蛋白的表达与肝外胆管癌的发生、发展及恶性行为密切相关 ,可作为判断肝外胆管癌恶性度和预后的指标。
Objective To study the significance of p16 protein expression in extrahepatic bile duct malignant tumors. Methods Immunohistochemical ABC method was used to detect the expression of p16 protein in 41 cases of extrahepatic cholangiocarcinoma and 12 cases of bile duct cysts. Results The positive expression rate of 1p16 protein in bile duct cysts was significantly higher than that of extrahepatic bile duct carcinoma. There was a significant difference between them. The positive expression rate of 2p16 protein was closely related to the degree of differentiation of extrahepatic cholangiocarcinoma, clinical stage (stages I-II and III-IV) and whether there was lymphatic metastasis (P < 0.05), but no expression of p16 protein was found. There was a correlation with pathological types of cholangiocarcinoma (P > 0.05). The survival time of 3p16 protein positive group was significantly longer than that of p16 protein negative group. Conclusion The expression of p16 protein is closely related to the occurrence, development and malignant behavior of extrahepatic cholangiocarcinoma. It can be used as an index to judge the malignancy and prognosis of extrahepatic cholangiocarcinoma.