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目的:探讨LINC01133/微小RNA(miRNA，miR)-205/谷胱甘肽过氧化物酶3(GPX3)轴对乳腺癌细胞增殖和迁移的影响及其机制。方法:收集河南省人民医院2018年1月到2020年1月手术摘除的129例乳腺癌和癌旁组织，采用荧光定量聚合酶链反应(PCR)分析LINC01133和miR-205表达水平；在MCF-7细胞建立LINC01133过表达和miR-205沉默细胞系，分别作为LINC01133组、lncRNA对照组、miR-205 KD组和miR对照组。分别采用细胞计数试剂盒(CCK-8)分析细胞增殖能力，采用Transwell分析细胞迁移能力；采用生物信息学分析LINC01133和miR-205关系及其靶蛋白；采用蛋白质印迹法(Western blot)分析靶蛋白表达水平。组间比较采用n t检验。n 结果:与癌旁组织LINC01133表达水平(1.12±0.15)比较，乳腺癌组织中LINC01133表达水平(0.57±0.10)显著下调，差异有统计学意义(n t＝3.011，n P<0.05)。与lncRNA对照组细胞LINC01133表达水平(1.05±0.11)比较，LINC01133组细胞LINC01133表达水平(2.48±0.21)显著增加，差异有统计学意义(n t＝4.108，n P<0.05)。与lncRNA对照组细胞吸光度值(2.29±0.20)比较，LINC01133组细胞吸光度值(1.54±0.16)显著下降，差异有统计学意义(n t＝3.025，n P<0.05)。与lncRNA对照组细胞迁移数量[(80.71±8.19)个]比较，LINC01133组细胞迁移数量[(43.77±6.47)个]显著下降，差异有统计学意义(n t＝2.810，n P<0.05)。生物信息分析显示LINC01133的靶miRNA是miR-205，miR-205的靶基因是GPX3，miR-205与GPX3 mRNA 3’端非编码区(3’UTR)区域存在碱基互补。与lncRNA对照组细胞GPX3蛋白表达水平(0.28±0.10)比较，LINC01133组细胞中GPX3蛋白表达水平(1.08±0.22)显著增加，差异有统计学意义(n t＝2.791，n P<0.05)。与miRNA对照组细胞GPX3蛋白表达水平(0.58±0.16)比较，miR-205 KD组细胞GPX3蛋白表达水平(1.98±0.24)显著增加，差异有统计学意义(n t＝3.019，n P<0.05)。n 结论:LINC01133在乳腺癌中呈低表达，通过miR-205/GPX3轴参与乳腺癌的增殖和迁移。“,”Objective:To investigate the effect of Effects of LINC01133/microRNA (miRNA, miR)-205/glutathione peroxidase 3 (GPX3) axis on the proliferation and migration of breast cancer cells and its molecular mechanism.Methods:129 cases of breast cancer and adjacent tissues from January 2018 to January 2020 were collected and the expression levels of LINC01133 and miR-205 were analyzed by fluorescence quantitative polymerase chain reaction (PCR); LINC01133 overexpression and miR-205 silencing cell lines were established in MCF-7 cells as LINC01133 group, lncRNA control group, miR-205 KD group and miRNA control group, respectively. Cell proliferation were analyzed by cell counting kit-8 (CCK-8) kit and cell migration was analyzed by Transwell. The relationship between LINC01133 and miR-205 and their target protein was analyzed by bioinformatics. The expression level of target protein was analyzed by Western blotting.Results:Compared with the expression level of LINC01133 (1.12±0.15), the expression level of LINC01133 in breast cancer tissues (0.57±0.10) significantly decreased (n t=3.011, n P<0.05). Compared with lncRNA control group (1.05±0.11), LINC01133 expression level in LINC01133 group (2.48±0.21), significantly increased (n t=4.108, n P<0.05). Compared with lncRNA control group (2.29±0.20), the cell absorbance of linc01133 group (1.54±0.16) significantly decreased (n t=3.025, n P<0.05). Compared with lncRNA control group [(80.71±8.19) cells], the number of cell migration in LINC01133 group [(43.77±6.47) cells,n t=2.810, n P<0.05]. Bioinformatics analysis showed that the target miRNA of LINC01133 was miR-205, and the target gene of miR-205 was GPX3. There was base complementary between miR-205 and GPX3 mRNA 3’untranslated regions (3’UTR) region. Compared with lncRNA control group (0.28±0.10), the expression level of GPX3 protein in LINC01133 group significantly increased (1.08±0.22,n t=2.791, n P<0.05). Compared with miRNA control group (0.58±0.16), the expression level of GPX3 protein in miR-205 KD group significantly increased (1.98±0.24,n t=3.019, n P<0.05).n Conclusion:LINC01133 is low expressed in breast cancer, and participates in the proliferation and migration of breast cancer through miR-205/GPX3 axis.