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To evaluate the antitumor effect of Octreotide on subcutaneous transplantation model of H22 cell line in mice and to explore the possible antitumor mechanisms of Octreotide.Methods Twenty male Kunming mice were enrolled into this study. They were randomized into two groups: Group A (experimental group) and Group B (control group). Each group contained 10 mice. H-22 cell line (2×10~6) was implanted subcutaneously into left armpit of each mice. Everyone in group A received Octreotide ( 100μg/kg/day×14 days) intraperitoneal injection just after implantation. Each mouse in group B received the same amount of saline solution intraperitoneal injection just after implantation. Subcutaneous tumor size was measured everyday. Fourteen days after implantation, all mice were killed, and the subcutaneous tmors were removed for immunohistochemical examination. VEGF, mierovessel density (MVD), PCNA, bcl2 were detected by immunohistochemistry.Results Mean size of subcutaneous tumors in Group A was significantly smaller than that in Group B 7 days and 14 days after implantation respectively (0.13±0.02cm~3 vs. 0.21±0.04cm~3, P<0.05; 0.36±0.11cm~3 vs. 0.72±0.11cm~3, P<0.01). The expression of VEGF and PCNA in Group B was significant higher than that in Group A (Ridit analysis, P<0.05). The expression of bcl-2 was also higher in Group B than in Group A, but without statistical significant difference (Ridit analysis, P>0.05). MVD in Group B was greater than that in group A, but without statistical significant difference (23.30±1.81 vs 30.60±3.41, P=0.075).Conclusion Octreotide has antitumor effect on subcutaneously transplanted H-22 cell line in mice through the mechanism of inhibiting cell proliferation and tumor angiogenesis.
To evaluate the antitumor effect of Octreotide on subcutaneous transplantation model of H22 cell line in mice and to explore the possible antitumor mechanisms of Octreotide.Methods Twenty male Kunming mice were enrolled into this study. They were randomized into two groups: Group A (experimental group Each group contained 10 mice. Each group contained 10 mice. Four days days after implantation, all mice were killed, and the subcutaneous tmors were removed. For immunohistochemical examination. VEGF, mierovessel density (MVD), PCNA, bcl2 were detected by immunohistochemistry.Results Mean size of subcutaneous tumors in Group A was Significantly smaller than that in group B 7 days and 14 days after implantation respectively (0.13±0.02cm~3 vs. 0.21±0.04cm~3, P<0.05; 0.36±0.11cm~3 vs. 0.72±0.11cm~3, P<0.01). The expression of VEGF and PCNA in Group B was significant higher than that in Group A (Ridit analysis, P<0.05). The expression of bcl-2 was also higher in Group B than in Group A, but without Compared with group A, but possessing significant difference (23.30±1.81 vs 30.60±3.41, P=0.075).Conclusion Octreotide has antitumor effect on subcutaneously Transplanted H-22 cell line in mice through the mechanism of inhibiting cell proliferation and tumor angiogenesis.