【摘 要】
：
The pre-mRNA processing factor Prp6 is an essential component of the U4/U6.U5 tri-small nuclear ribonucleoprotein (snRNP). In a previous study, mutations were identified in the PRP6 ortholog in four suppressors of Fgprp4 that was deleted of the only kinas
【机 构】
：
NWAFU-PU Joint Research Center/State Key Laboratory of Crop Stress Biology for Arid Areas,College of
论文部分内容阅读
The pre-mRNA processing factor Prp6 is an essential component of the U4/U6.U5 tri-small nuclear ribonucleoprotein (snRNP). In a previous study, mutations were identified in the PRP6 ortholog in four suppressors of Fgprp4 that was deleted of the only kinase FgPrp4 among the spliceosome components in the plant pathogenic fungus Fusarium graminearum. In this study, we identified additional suppressor mutations in FgPrp6 and determined the suppressive effects of selected mutations. In total, 12 mutations of FgPRP6 were identified in 20 suppressors of Fgprp4 by sequencing analysis. Whereas three mutation sites are in the linker region of FgPrp6, seven are in the first two HAT repeats. RNA-seq analysis showed that suppressor mutations on different sites caused different splicing efficiency recovery. The suppressive effects of E308K and R230H were verified. Similar to human and fission yeast, the FgPrp6 was phosphorylated by the FgPrp4 kinase. Interestingly, the conserved Prp4-phosphorylation sites T261, T219&T221, and predicted phosphorylation sites T199&T200 on FgPrp6 were dispensable for the function of FgPrp6 in hyphal growth and sexual reproduction but important in plant infection. They are required for the infectious growth of F. graminearum in wheat lemma. RNA-seq analysis of the wheat lemma infected with Fgprp6/FgPRP6Δ199–221-GFP or Fgprp6/FgPRP6Δ250–262-GFP showed that 28 and 35％ introns had splicing defects, respectively, which may be responsible for their defects in plant infection.
其他文献
为实现农机装备的自动转向,采用直流电机经过齿轮减速器力矩放大后驱动全液压转向器的方法,设计一款高度集成的农机自动转向集成装置系统,包括自动转向控制结构、控制器电路及其车轮转向精确伺服控制算法,控制器采用飞思卡尔车规级芯片,并考虑在农机在未点火发动状态下的不安全转向情况,使得控制逻辑更加安全可靠.通过Matlab建立农机前轮的电控转向液压模型,开展不同转向角度信号的仿真试验,验证本文控制算法的响应特性.将自动转向集成装置安装在高地隙植保机器人上开展田间实车试验,试验结果表明,车轮转角控制误差小于0.2°,超
以冷榨法提取的茶油毛油、不同精炼阶段获得的脱酸油、脱色油、脱蜡油及成品茶油为研究材料,对比分析了各阶段茶油的酸价、过氧化值、自由基清除能力的变化规律.结果表明:5种茶油中,总酚含量以毛油最高,碱炼脱酸后的脱酸油总酚含量明显下降,而脱蜡后的脱蜡油总酚浓度上升了63.5％,原因可能是低温条件有助于茶油中酚类物质结构恢复,在无碱液影响下,酚类物质受破坏程度大大减少,总酚含量升高;过氧化值以脱酸油最低,脱酸油的过氧化值下降了29.3％;酸价以毛油最高,碱炼脱酸后,脱酸油的酸价下降最显著,比毛油下降了85.3％;茶
为探索豫南烟区中部叶不同成熟度烟叶的适宜烘烤工艺,实现烟农增收和烟叶品质提升的目的,以云烟87中部叶为材料,首先对烟叶成熟度进行了分类,然后针对不同成熟度的烟叶分别进行烘烤工艺优化.结果表明:对鲜烟叶进行成熟度分类,该批次鲜烟叶中适熟与低成熟度烟叶的比例为3:1;通过对不同成熟度烟叶分类装炕精准烘烤(低成熟度烟叶采用T1工艺,主要是延长烘烤预变黄过程、提升烟叶的后熟效应;适熟烟叶采用T3工艺,主要是定色前降低烟叶水分,并延长定色烘烤时间),其烤后烟叶综合的上等烟比例和均价分别较常规烘烤提升8.20％和8.
通过田间调查及生理生化测试,分析比较了紫甘薯浙紫薯1号野生型及其芽变突变体植株的植物学特征、光合特性、农艺性状、产量及块根品质的差异.结果表明:与野生型相比,突变体茎粗加大,茎长和节间长度显著变短,生长势旺盛;野生型茎和根系为紫色,突变体茎和根系颜色分别为绿色和白色;突变体叶片净光合速率高于野生型;突变体单株结薯数减少,单株产量和单位面积产量显著增加;野生型薯皮、薯肉均为紫色,其花青素含量分别为666.0、536.7 mg/kg,突变体薯皮和薯肉颜色分别为白色和黄色,其花青素含量分别为15.3、11.2
为了解和比较红白两种颜色藜麦主要营养成分的含量,本文采用红、白两色藜麦为原料,采用直接干燥法、高温灼烧法、索氏提取法及凯氏定氮法四种方法分别检测两种藜麦的水分含量、灰分含量、脂肪含量和蛋白质含量.结果表明,白色藜麦的水分含量在7.52％～8.33％之间,灰分含量在2.38％～2.47％之间,脂肪含量在3.71％～3.89％之间,蛋白质含量在20.88％～20.95％之间;红色藜麦的水分含量在8.24％～9.15％之间,灰分含量在2.05％～2.19％之间,脂肪含量在4.19％～4.20％之间,蛋白质含量在
为了加强野生黄栀子资源的保护与利用,促进黄栀子产业的健康发展,从湖南、湖北不同地方收集13份野生黄栀子资源进行了农艺性状比较和主要性状的相关性分析.结果表明:13份野生黄栀子资源的株型、叶片性状、开花数、结果数、果实性状等主要农艺性状差异明显;黄栀子资源4号的综合性状优于其他资源,其秋季复花量最多,春季开花量较多,而且其株型松散,果型大,果色好,综合观赏性能优,用途广,是用于观赏的好材料;黄栀子资源13号的株型紧凑,叶质硬小,叶色深,是做造型和绿篱的良好材料;黄栀子资源1、2、4、6、8、9号的秋季复花量
棉花夏播短季轻简化栽培是入夏后播种,简化生产程序,全生育期控制在150 d以内的栽培模式,其关键技术包括:①推迟播种,改春播为夏播;②适当密植,发挥群体效应;③选用良种,提高播种质量;④平衡施肥,减少氮肥用量;⑤全程化调,适时打顶,塑造理想株型;⑥适时催熟脱叶,促进集中吐絮.这种栽培模式具有生产周期短、生产环节少、小个体大群体结构、吐絮集中、纤维品质一致性好等特点和优势,可大幅降低棉花生产成本、提高植棉效益,并为棉田耕作制度的根本性变革和棉花全程机械化生产提供基础,具有较好的推广应用前景.
莲子别名藕实、莲蓬子、莲米,是睡莲科植物莲的干燥成熟种子.莲子中含有丰富的营养成分,同时又极具医用价值,已列入我国国家卫生健康委员会公布的药食两用名单中.成熟的莲子剥壳后可以直接食用,也可制成莲子汁,而干制莲子常制作成药材、点心、零食、莲蓉等.莲子粉中主要含有丰富的淀粉、脂肪和蛋白质,还有生物碱和黄酮类化合物,有较高的研究价值.本文对莲子粉的主要成分和作用进行综述分析,为莲子粉产品制作及工艺提供参考依据.
“三农”问题事关我国社会、经济发展质量,事关人民幸福感、获得感和安全感,但农业专业型人才的短缺成为我国农业发展和乡村振兴的一大阻力.目前,我国农业从业人员的整体素质不高,同时,农村人才流失严重,农业从业人员结构老龄化加剧,培养高学历、懂生产、懂管理的综合性农业一线人才显得十分紧迫.开展农业科普教育,引导青少年学生正确认识农业,树立正确的择业观念,在农业储备人才培养中尤为重要.
Grapevine growing areas are increasingly affected by drought, which has greatly limited global wine production and quality. DEAD-box is one of the largest subfamilies of the RNA helicase family, and its members play key roles in the growth and development