A Comparison Between Northern Blotting and Quantitative Real-Time PCR as a Means of Detecting the Nu

• 作 者: GAN Yin-bo ZHOU Zhong-jing AN
• 机 构: CollegeofAgricultureandBiotechnology,DepartmentofBiologicalSciences
• 出 处: 中国农业科学：英文版
• 发表日期: 2011年3期
• 关键词: 聚合酶链反应 基因表达 营养调控 实时定量 印迹杂交 检测结果 拟南芥 NORTHERN杂交 Arabidopsis thaliana MADS-box n
• 基金项目: supported by the Fundamental Research Funds for the Central Universities of China（2009QNA6023）, the International Scientific and Technological Cooperation Project of Ministry of Science and Technology

Quantitative real-time PCR (qRT-PCR) has become a routine and robust technique for measuring the expression of genes of interest, validating microarray experiments and monitoring biomarkers. However, concerns have been raised over the accuracy of qRT-PCR