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To generate monoclonal anti-idiotypic antibodies(mAb2)against avian influenza virus subtype H9(H9 AⅣ),BALB/c mice were immunized with purified chicken anti-H9-AⅣ IgG and the splenocytes of immunized mice werefused with myeloma cells NS-1.Hybridoma cells were screened by indirect enzyme-linked immunosorbent assayswith both chicken and rabbit anti-H9-AⅣ IgG as coating antigens.One hybridoma cell clone secreting monoclonalantibody against idiotypes shared by both chicken and rabbit anti-H9-AⅣ IgG was established.Experimentsdemonstrated the mAb2 was able to inhibit the binding of hemagglutinin to anti-H9-AⅣ IgG and to inducechickens to generate hemagglutination inhibition antibodies,indicating this anti-species-sharing-idiotypic antibodybore the internal image of hemagglutinin on avian influenza virus.Cellular & Molecular Immunology.2005;2(2):155-157.
To generate monoclonal anti-idiotypic antibodies (mAb2) against avian influenza virus subtype H9 (H9 AIV), BALB / c mice were immunized with purified chicken anti-H9-AIV IgG and the splenocytes of immunized mice werefused with myeloma cells NS-1. Hybridoma cells were screened by indirect enzyme-linked immunosorbent assays with both chicken and rabbit anti-H9-AIV IgG as coating antigens. One hybridoma cell clone secreting monoclonalantibody against idiotypes shared by both chicken and rabbit anti-H9-AIV IgG was established. Experiments the mAb2 was able to inhibit the binding of hemagglutinin to anti-H9-AIV IgG and to inducechens to generate hemagglutination inhibition antibodies, indicating this anti-species-sharing-idiotypic antibodybore the internal image of hemagglutinin on avian influenza virus. Cellular & Molecular Immunology. 2005; 2 (2): 155-157.