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不同糖源诱导产生的愈伤组织具有一定的后效应，一般在诱导阶段生长较慢的愈伤组织当转入同一培养基上培养时则表现出优势。以可溶性淀粉、蔗糖和麦芽糖为糖源的培养基上均可诱导棉花花药愈伤组织获得了胚胎发生和胚状体；葡萄糖虽有利于棉花花药愈伤组织生长，但却没能诱导胚胎发生和胚状体的形成。乳糖即不利于愈伤组织的生长，又不利于胚状体的形成。半乳糖作为糖源，愈伤组织虽能增殖，但生长速度极慢。山梨糖不能被棉花花药愈伤组织吸收，故以山梨糖为糖源时愈伤组织极易褐化死亡。甘露醇的添加促进了葡萄糖诱导形成胚性愈伤组织和胚状体。
Callus induced by different sugar sources has some post-effects. Generally, the callus, which grows slowly during the induction phase, shows advantages when it is transferred to the same culture medium. Induction of embryogenesis and embryoid formation on anther callus of cotton by using soluble starch, sucrose and maltose as sugar sources. Glucose, although conducive to cotton anther callus growth, failed to induce embryogenesis and Embryoid body formation. Lactose is not conducive to the growth of callus, but also not conducive to the formation of embryoid bodies. Galactose as a sugar source, although callus proliferation, but the growth rate is very slow. Sorbose can not be absorbed by the cotton anther callus, so sorbose is a source of sugar when the callus easily browning death. The addition of mannitol promoted glucose-induced formation of embryogenic callus and embryoid bodies.