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目的初步探索职业性慢性铅中毒患者T淋巴细胞亚群及Th淋巴细胞因子的变化特点。方法对23例职业性慢性铅中毒患者(铅中毒组)及20例健康非职业铅接触成人(对照组)采用流式细胞技术检测外周静脉血淋巴细胞CD_3、CD_4、CD_8的表达,同时通过流式细胞微球芯片捕获技术检测血浆IL-2、IL-4、IL-6、IL-10、TNF-αt、IFN-γ的浓度。结果铅中毒患者外周静脉血CD_4相对百分比(32.68%±11.54%)及CD_4/CD_8比值(0.89±0.39)与对照组比较,明显下降,差异有统计学意义(P<0.05);血浆IL-2浓度为(2.00±0.68)pg/ml,与对照组比较明显上升,差异有统计学意义(P<0.05);血浆IL-10和IFN-γ浓度分别为(1.83±0.85)pg/ml和(3.42±0.85)pg/ml,与对照组比较明显减低,差异有统计学意义(P<0.05)。结论T淋巴细胞亚群及细胞因子对评价铅免疫毒性有进一步研究价值。
Objective To explore the characteristics of T lymphocyte subsets and Th lymphocyte factor in patients with occupational chronic lead poisoning. Methods The expression of CD_3, CD_4 and CD_8 in peripheral blood lymphocytes was detected by flow cytometry in 23 cases of chronic lead poisoning (lead poisoning group) and 20 healthy non-occupational lead exposure adults (control group) The concentration of IL-2, IL-4, IL-6, IL-10, TNF-αt and IFN-γ in plasma was detected by the cell microarray chip capture technique. Results Compared with the control group, the relative percentages of CD_4 in peripheral blood (32.68% ± 11.54%) and CD_4 / CD_8 (0.89 ± 0.39) in lead poisoning patients were significantly lower than those in control group (P <0.05) (2.00 ± 0.68) pg / ml, which was significantly higher than that of the control group (P <0.05). The concentrations of plasma IL-10 and IFN-γ were (1.83 ± 0.85) pg / ml and 3.42 ± 0.85) pg / ml, which was significantly lower than that of the control group (P <0.05). Conclusion T lymphocyte subsets and cytokines are of further value in the evaluation of lead immunotoxicity.