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本文在前文~[2]的基础上进一步以MCN和DNaseⅠ为探针研究大鼠脑神经元终末分化后不同生理时期染色质构象,结果表明:MCN酶解DNA产物PAGE显示脑老化过程大脑皮层及小脑神经元染色质核小体单体DNA分别保持在176bp和215bp水平,核小体连接DNA长度存在组织差异,但不受老化影响;<2>DNaseⅠ酶解DNA产物PAGE显示各年龄组大脑皮层及小脑神经元染色质DNA存在10bp间隔重复结构和相同的泳动区带分布特征,提示脑老化中染色质具有稳定的B型双螺旋结构和一致的螺线管卷曲形式。染色质DNaseⅠ降解率随年龄增加而降低,提示老化导致活性染色质区域减少,老化过程脑神经元染色质构象改变成为其转录功能减退的结构基础。
Based on the previous article [2] on the basis of further MCN and DNase Ⅰ probe study of rat brain neurons after the end of the differentiation of chromatin conformation at different physiological stages, the results showed that: MCN enzymatic DNA product PAGE showed brain aging In the process of cerebral cortex and cerebellar neuron, the monomer DNA of chromatin nucleosomes remained at 176bp and 215bp, respectively. The length of nucleosome ligated DNA was different but not influenced by aging. <2> In the age group, the 10 bp repeat structure and the same motile zone distribution existed in the chromatin of cerebral cortex and cerebellar neurons, indicating that chromatin has a stable B-type double helix structure and consistent solenoid curl pattern in brain aging. The degradation rate of chromatin DNase Ⅰ decreased with age, suggesting that the aging led to the reduction of the active chromatin region, and the chromatin conformational changes of the neurons in the aging process became the structural basis of its transcriptional decline.