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目的 :探讨 c- myc基因的反义寡核苷酸 (ASODN)在涎腺粘液表皮样癌基因治疗中的作用。方法 :人工合成与 c- myc基因第二外显子翻译起始区序列互补的寡核苷酸 ,处理培养的人涎腺粘液表皮样癌 MEC- 1细胞。应用 MTT比色法观察其对细胞增殖的影响 ;流式细胞仪检测对细胞周期的影响 ;并采用免疫组化法检测 C- MYC蛋白的表达。结果 :c- myc反义寡核苷酸能抑制粘液表皮样癌 MEC- 1细胞的增殖 ,抑制作用具有浓度及时间依赖性。 5 0 %抑制浓度 (IC5 0 )为 8.2 μmol/ L。用浓度为 10 μmol/ L 的 c- myc ASODN处理 72 h,对 MEC- 1细胞的抑制率达 5 9%。c- myc ASODN抑制 MEC- 1细胞从 G1 期进入 S期 ;并能抑制 C- MYC蛋白的表达。结论 :c- myc反义寡核苷酸对粘液表皮样癌 MEC- 1细胞有特异性抑制作用
Objective: To investigate the role of antisense oligonucleotide (c-myc) antisense oligonucleotide (ASODN) in mucoepidermoid carcinoma gene therapy in salivary glands. Methods: Human salivary gland mucoepidermoid carcinoma MEC-1 cells were treated with oligonucleotides complementary to the translation initiation region of exon 2 of c-myc gene. MTT colorimetric assay was used to observe the effects on cell proliferation. Flow cytometry was used to detect the cell cycle. The expression of C-MYC protein was detected by immunohistochemistry. Results: The c-myc antisense oligonucleotide could inhibit the proliferation of mucoepidermoid carcinoma MEC-1 cells in a concentration-and time-dependent manner. The 5 0% inhibitory concentration (IC 50) was 8.2 μmol / L. Treatment with c-myc ASODN at a concentration of 10 μmol / L for 72 h resulted in a 59% inhibition of MEC-1 cells. c-myc ASODN inhibits the entry of MEC-1 cells from G1 phase to S phase and inhibits the expression of C-MYC protein. Conclusion: c-myc antisense oligonucleotides have specific inhibitory effect on mucoepidermoid carcinoma MEC-1 cells