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目的观察不同浓度、不同时间P物质(substance P,SP)对骨髓间充质干细胞源性成骨细胞矿化的影响,并用SP非特异性的阻滞剂和NK1受体特异性的阻滞剂进行干预,从而推测其作用细胞的靶受体。方法 10只雄性6周龄SD大鼠制作脊髓损伤模型。从模型的胫骨、股骨提取骨髓间充质干细胞(mesenchymal stemcells,MSCs)进行成骨细胞的诱导培养,用第二代细胞进行实验。施加不同浓度的SP,对照组不加任何处理因素,在不同的时间点观察骨髓MSCs源性成骨细胞的矿化。并用SP、Spantide(SP的抑制剂)、L703606(NK1受体的抑制剂)进行配伍作用,观察骨髓MSCs源性成骨细胞的矿化。结果不同浓度的SP对细胞的矿化有不同程度的抑制作用,10~(-8)M浓度的抑制作用最强,矿化结节面积较对照组平均减少了38.3%(P<0.01),随时间的延长抑制作用越强(3W:-34.9%,P<0.01)。细胞矿化的抑制可以被Spantide和L703606所逆转,与SP组比较两组矿化分别升高了45.3%和50.5%。结论 SP通过NK1受体对成骨细胞的矿化产生影响。SP抑制了成骨细胞的矿化,使成熟的成骨细胞减少,从而引起脊髓损伤后骨质疏松的发生。
Objective To observe the effect of substance P (SP) on the mineralization of mesenchymal stem cells derived osteoblasts at different concentrations and different times, and use SP nonspecific blockers and NK1 receptor specific blockers Interference, which speculated that the role of the target cell receptors. Methods Ten male 6-week-old SD rats were used to make spinal cord injury model. Osteoblasts were induced from mesenchymal stem cells (MSCs) from the tibia and femur of the model, and the second generation of cells were used for experiments. Different concentrations of SP were applied to the control group without any treatment, and bone mineralization of MSCs derived osteoblasts was observed at different time points. The combination of SP, Spantide (inhibitor of SP) and L703606 (inhibitor of NK1 receptor) was used to observe the mineralization of bone marrow MSCs derived osteoblasts. Results Different concentrations of SP inhibited the mineralization of cells to a certain extent. The inhibitory effect of 10 -8 M concentration was the strongest, and the mineralized nodule area was reduced by 38.3% (P <0.01) on average compared with the control group. With the extension of time the more inhibitory effect (3W: -34.9%, P <0.01). The inhibition of cell mineralization could be reversed by Spantide and L703606, which increased by 45.3% and 50.5% respectively compared with SP group. Conclusion SP affects the mineralization of osteoblasts through NK1 receptors. SP inhibits the mineralization of osteoblasts, reducing the number of mature osteoblasts, resulting in the occurrence of osteoporosis after spinal cord injury.