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目的:用正交试验法考察甘木通总黄酮的最佳提取条件。方法:以芦丁为对照品建立标准曲线方程用于测定甘木通总黄酮含量,以乙醇为溶剂分别考察温度、乙醇浓度、提取时间、药材/溶剂比例及提取次数等因素对甘木通总黄酮提取率的影响,据此设计四因素三水平正交试验,系统考察甘木通总黄酮的最佳提取条件。结果与结论:药材与70%乙醇按照1:20的比例,在90℃回流提取1h共2次,甘木通总黄酮提取率可达93.27%。
OBJECTIVE: To study the optimum extraction conditions of total flavonoids from Ganmu tong by orthogonal test. Methods: The calibration curve of rutin was used to establish the standard curve equation for the determination of the content of total flavonoids in Ganmu tong, and the effects of temperature, ethanol concentration, extraction time, ratio of medicinal materials to solvent and extraction times on the extraction of total flavonoids Rate, according to the design of four factors and three levels of orthogonal test, systematic investigation of the optimum extraction conditions of total flavonoids Ganmutong. RESULTS AND CONCLUSION: The extraction rate of total flavonoids from Ganmu tong was up to 93.27% by refluxing and extracting for 1 hour at 90 ℃ for 1 hour with the ratio of 1:20 and 70% ethanol.