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本文介绍了偏最小二乘法(PLS)在多组分分光光度分析中的基本原理、方法和计算,并用该法测定了复方磺胺林片的含量。磺胺林(SMPZ)和甲氧苄氨嘧啶(TMP)的平均回收率分别为100.0±0.13%和100.0±0.46%(95%置信度),变异系数分别为0.13%和0.46%。PLS法为多组分分析提供了一种新的测定方法。
This paper introduces the principle, method and calculation of Partial Least Squares (PLS) in multi-component spectrophotometric analysis. The content of compound sulfamethoxazole tablets is also determined by this method. The average recoveries of sulfamethoxazole (SMPZ) and trimethoprim (100 mg) were 100.0 ± 0.13% and 100.0 ± 0.46% (95% confidence) with coefficients of variation of 0.13% and 0.46%, respectively. PLS method provides a new method for multi-component analysis.