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目的研究电场方向对电穿孔技术促进咖啡因经皮渗透的影响。方法应用水平双室扩散池,Ag-Ag/AgCl电极和人尸体皮肤,进行咖啡因的经皮渗透实验,考察电场方向对电穿孔(指数衰减型脉冲,脉冲幅度为350 V,脉冲率为4 pulses·min-1,脉冲数为25,电容为22μF )促渗作用的影响,并与离子导入(0.25 mA·cm-2,持续4小时)进行比较。结果 1)电穿孔与离子导入均可显著增加咖啡因的经皮渗透速率和累积渗透量。2)阳极离子导入(电场方向为从供应室到接受室)的促渗作用显著大于阴极离子导入(电场方向为从接受室到供应室)的促渗作用。3)阳极电穿孔的促渗作用与阴极电穿孔的促渗作用无显著差异。4)阳极离子导入的促渗作用显著大于阳极电穿孔及阴极电穿孔的促渗作用。结论电场方向对离子导入的促渗作用有显著影响,而对电穿孔的促渗作用没有影响。
Objective To study the influence of electric field direction on the transdermal penetration of caffeine by electroporation. Methods Percutaneous transdermal infusion of caffeine was performed using horizontal double chamber diffusion cells, Ag-Ag / AgCl electrodes and human cadaver skin. Electroporation (exponential decay pulse, pulse amplitude 350 V, pulse rate 4 pulse · min-1, a pulse number of 25, a capacitance of 22 μF), and compared with the iontophoresis (0.25 mA · cm -2 for 4 hours). Results 1) Both electrostimulation and iontophoresis could significantly increase the transdermal permeation rate and cumulative permeation of caffeine. 2) The effect of anodic ion introduction (the direction of the electric field is from the supply chamber to the receiving chamber) is significantly greater than that of the cathodic ion introduction (the electric field direction is from the receiving chamber to the supplying chamber). 3) There was no significant difference between the enhancement effect of electroporation on the anode and the enhancement of electroporation on the cathode. 4) The iontophoresis effect of anodic ion implantation was significantly greater than that of anodic electroporation and cathodic electroporation. Conclusions The direction of electric field has a significant effect on the promoting effect of iontophoresis, but has no effect on the promoting effect of electroporation.