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通过RACE技术从花生叶片cDNA中克隆到了UDP-葡萄糖基转移酶的基因,命名为AhUGT83A1-like(GenBank注册号为KF411463)。该基因全长为1 530bp,ORF为1 380bp,编码460个氨基酸。序列比对与进化分析结果表明,该序列有保守的UDPGT结构域,并且与其它植物的UGT蛋白同源性较高。荧光定量PCR结果表明,AhUGT83A1-like基因在高盐和低温处理的花生根和叶片中均上调表达;在干旱处理时,根中表达也受到显著上调,但叶片中表达量则有明显下降。以上结果表明AhUGT83A1-like可能参与了花生对干旱、低温和高盐的抗性调控。ABA处理结果表明,AhUGT83A1-like在花生根中对ABA响应明显,但在叶片中对ABA没有明显响应,表明该基因在花生根中对非生物胁迫的调控可能是以依赖ABA的方式发挥作用的。
The UDP-glucosyltransferase gene was cloned from peanut leaf cDNA by RACE technology and named AhUGT83A1-like (GenBank accession number KF411463). The full length of this gene was 1 530bp and the ORF was 1 380bp, encoding 460 amino acids. Sequence alignment and phylogenetic analysis showed that this sequence has a conserved UDPGT domain and high homology with UGT proteins of other plants. Fluorescence quantitative PCR results showed that AhUGT83A1-like gene was up-regulated in roots and leaves of peanut treated with high salinity and low temperature, but also significantly up-regulated in roots while its expression was significantly decreased in drought treatment. The above results indicated that AhUGT83A1-like may be involved in the regulation of peanut resistance to drought, low temperature and high salt. The results of ABA showed that AhUGT83A1-like had obvious ABA response in the peanut root but no obvious response to ABA in the leaves, indicating that the regulation of abiotic stress on this gene may play an ABA-dependent role .