论文部分内容阅读
目的观察二甲双胍对大鼠肾小球系膜细胞(MCs)核因子-κB(NF-κB)、单核细胞趋化蛋白-1(MCP-1)、细胞间黏附分子-1(ICAM-1)表达及合成的影响,探讨其对肾脏的保护机制。方法体外培养MCs,随机分为正常糖组(NG)、高糖组(HG)及高糖+不同浓度二甲双胍组(M1、M2、M3)。培养48 h后收集各组细胞及上清液,采用荧光实时定量聚合酶链式反应(Real-time PCR)法检测细胞NF-κB、MCP-1及ICAM-1的mRNA含量;采用Western blot法检测细胞NF-κBp65及ICAM-1蛋白表达水平;采用ELISA法检测上清液中MCP-1蛋白浓度。结果①MCs可表达NF-κB、MCP-1和ICAM-1;②与NG组比较,HG组MCs NF-κB、MCP-1、ICAM-1的mRNA表达增强,NF-κBp65、ICAM-1及细胞上清液中MCP-1蛋白含量增高(P<0.05);③经二甲双胍干预后,高糖培养的MCs NF-κB、MCP-1及ICAM-1表达合成显著降低(P<0.05)。结论二甲双胍可抑制MCs NF-κB、MCP-1和ICAM-1的表达及合成,该作用可能与其肾脏保护机制有关。
Objective To observe the effects of metformin on the expression of NF-κB, MCP-1, ICAM-1 in rat mesangial cells (MCs) Expression and synthesis, to explore its mechanism of protection of the kidneys. Methods MCs were cultured in vitro and randomly divided into normal glucose group (NG), high glucose group (HG) and high glucose + metformin group (M1, M2, M3). After cultured for 48 h, the cells and supernatants of each group were collected, and the mRNA levels of NF-κB, MCP-1 and ICAM-1 were detected by Real-time PCR with fluorescence quantitative real-time PCR. The expression of NF-κBp65 and ICAM-1 protein in the cells was detected. The concentration of MCP-1 protein in the supernatant was detected by ELISA. Results ①MCs could express NF-κB, MCP-1 and ICAM-1. ② Compared with NG group, the mRNA expression of NF-κB, MCP-1 and ICAM-1 increased The content of MCP-1 in the supernatant increased (P <0.05). ③ The expression of NF-κB, MCP-1 and ICAM-1 in MCs cultured with high glucose was significantly decreased after metformin intervention (P <0.05). Conclusion Metformin can inhibit the expression and synthesis of NF-κB, MCP-1 and ICAM-1 in MCs, which may be related to the mechanism of renal protection.