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为更好地利用生防菌控制青枯病危害,从不同地区的土壤中分离到569株细菌菌株,筛选到3株对5种不同生化型青枯劳尔氏菌Ralstonia solanacearum具有较强拮抗活性的菌株,其中菌株BS2004的拮抗活性最强。以BS2004的菌悬液为对照,分别测定无菌滤液、蛋白酶K及高温热处理后拮抗物质抑菌活性的变化。结果显示,蛋白酶K及高温热处理后,该菌的抑菌活性显著降低,表明其主要抑菌成分为蛋白类物质。在设施栽培条件下用生防菌BS2004菌悬液处理番茄植株,能有效控制番茄青枯病的发生,防治效果达66.75%,同时还发现,重新分离得到的青枯菌菌体数明显受到生防菌的抑制。通过对BS2004的形态、生理生化特征、脂肪酸鉴定、16S rDNA序列等进行分析,该菌株被鉴定为解淀粉芽孢杆菌Bacillus amyloliquefaciens。
In order to make better use of biocontrol microbes to control the bacterial wilt, 569 strains of bacteria were isolated from soil in different regions, and 3 strains of Ralstonia solanacearum were screened for their antagonistic activities against 5 different biochemical species of Ralstonia solanacearum Strain BS2004, of which the antagonistic activity strongest. The bacterial suspension BS2004 as a control, were measured sterile filtrate, proteinase K and high temperature heat treatment antagonist changes in antibacterial activity. The results showed that the bacteriostatic activity of the bacterium was significantly decreased after protease K and high temperature heat treatment, indicating that its main bacteriostatic component is proteinaceous material. Under the condition of cultivation, the tomato plants were treated with the suspension of biocontrol strain BS2004, which could effectively control the occurrence of tomato bacterial wilt. The control effect was 66.75%. At the same time, it was also found that the number of newly isolated R. solanacearum cells was significantly increased Antibacterial inhibition. The strain BS2004 was identified as Bacillus amyloliquefaciens by morphological, physiological and biochemical characteristics, fatty acid identification, 16S rDNA sequence and so on.