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[Objective] The research aimed to optimize the extraction process of total flavonoids in the leaves of Poacynum hendersonii (Hook. f.) Woodson from Qinghai. [Method] The influence of different ethanol volume, extracting temperature, treatment time, solid-liquid ratio, extracting time, mesh, ultrasound power, pH on ultrasonic extraction of total flavonoids in the leaves of Poacynum hendersonii (Hook. f.) Woodson were studied. The extracting technology was optimized on the basis of single factor experiments through orthogonal design. [Result] The results showed the optimal conditions for the extraction of total flavonoids in Poacynum hendersonii (Hook. f.) Woodson. leaves were as follows: ethanol concentration 65%, extraction temperature 50 ℃, treatment time 2 h, ratio of material to solvent 1∶15, extraction times 3, mesh 20, ultrasonic power 100 W and pH value 9. Under the optimized conditions, the extraction rate of total flavonoids was the highest. [Conclusion] The study can provide the base data for deeper exploration and utilization of Poacynum hendersonii (Hook. f.) Woodson. resources.
[Objective] The research aimed to optimize the extraction process of total flavonoids in the leaves of Poacynum hendersonii (Hook. F.) Woodson from Qinghai. [Method] The influence of different ethanol volume, extracting temperature, treatment time, solid-liquid ratio , extracting time, mesh, ultrasound power, pH on ultrasonic extraction of total flavonoids in the leaves of Poacynum hendersonii (Hook. f.) Woodson were studied. The extracting technology was optimized on the basis of single factor experiments through orthogonal design. [Result ] The results showed the optimal conditions for the extraction of total flavonoids in Poacynum hendersonii (Hook. F.) Woodson. Leaves were as follows: ethanol concentration 65%, extraction temperature 50 ° C, treatment time 2 h, ratio of material to solvent 1 : 15, extraction times 3, mesh 20, ultrasonic power 100 W and pH value 9. Under the optimized conditions, the extraction rate of total flavonoids was the highest. [Conclusion] The study can provi de the base data for deeper exploration and utilization of Poacynum hendersonii (Hook. f.) Woodson. resources.