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本文主要通过荧光探针结合流式细胞术,探讨了表没食子儿茶素没食子酸酯(EGCG)铜离子络合物(ECC)对结肠癌细胞(RKO 细胞)内活性氧水平的影响,并进一步从分子水平阐述了其影响机制。结果表明,5~10 mg/L的ECC能够显著增强胞内活性氧水平,并且随着ECC水平的增加,细胞周期主要阻止于G2/M期。另外,胞内黄嘌呤氧化酶活性呈降低趋势,并且超氧化物岐化酶-1活性并没有随着其基因水平的增强而显著增强;但过氧化氢酶虽然基因水平有了较大的提升,其在胞内的生物活性却呈现出显著性剂量依赖性降低。因此,胞内活性的提升可能主要归因于胞内活性氧(过氧化氢)未能及时被活性降低的过氧化氢酶清除所致。
In this paper, the effects of epigallocatechin gallate (EGCG) copper ion complex (ECC) on reactive oxygen species in colon cancer cells (RKO cells) were investigated by fluorescence probe combined with flow cytometry and further From the molecular level of its impact mechanism. The results showed that ECC of 5 ~ 10 mg / L could significantly increase intracellular reactive oxygen species (ROS), and with the increase of ECC level, the cell cycle mainly stopped in G2 / M phase. In addition, intracellular xanthine oxidase activity decreased, and superoxide dismutase-1 activity did not increase significantly with its gene level; but catalase gene level has been greatly improved , But its intracellular bioactivity showed a significant dose-dependent decrease. Therefore, the enhancement of intracellular activity may be mainly attributed to the intracellular reactive oxygen species (hydrogen peroxide) failed to be promptly eliminated by catalase with reduced activity.