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目的 :探讨体外高糖环境对大血管内皮细胞产生转化生长因子 -β(TGF-β)的影响。方法 :分别用含不同浓度葡萄糖 (12 .5 m M、2 5 m M、5 0 m M D-葡萄糖 )、高糖加肿瘤坏死因子 -α(TNF-α)和高糖加胰岛素的 10 %小牛血清 RPMI16 40液培养 SD大鼠主动脉血管内皮细胞 48h,取上述培养液分别用生物学方法测其 TGF-β1 水平 ,采用放射免疫法测其内皮素 (Endothelin ET)的水平。结果 :高糖环境下血管内皮细胞分泌的总 TGF-β1 (总 TGF-β1 含量为 40 6± 2 9vs189± 2 3pg/ ml,P<0 .0 5 )及活性 TGF-β1 (活性 TGF-β1 含量为 16 1± 2 4vs 39.5± 8pg/ ml,P<0 .0 5 )明显增加。TNF-α可以加强高糖的这种刺激作用 ,而胰岛素处理可以抑制这种作用。结论 :TGF-β可能参与了糖尿病大血管并发症的发生发展
Objective: To investigate the effect of high glucose in vitro on transforming growth factor-β (TGF-β) production by large vascular endothelial cells. Methods: The rats were randomly divided into three groups: control group (n = 10) containing different concentration of glucose (12.5 m M, 25 m M, 50 m D-glucose), high glucose plus TNF-α and high- Bovine serum RPMI1640 liquid culture of aortic vascular endothelial cells of SD rats 48h, respectively, the above culture medium were measured by the biological method of TGF-β1 levels by radioimmunoassay the level of endothelin (Endothelin ET). RESULTS: The total TGF-β1 (total TGF-β1 content of 40 6 ± 29 vs189 ± 23 pg / ml, P <0.05) and active TGF-β1 Content of 16 1 ± 2 4 vs 39.5 ± 8 pg / ml, P <0.05) increased significantly. TNF-α can enhance this stimulation of high glucose, and insulin treatment can inhibit this effect. Conclusion: TGF-β may be involved in the development of diabetic macrovascular complications