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细胞微囊化共培养在细胞诱导分化、细胞增殖调节和提高细胞代谢物产量方面是一种很有价值的技术。两种微囊化细胞的可视识别和分离在共培养过程中有重要作用。作为磁性介质,顺磁四氧化三铁纳米粒(Fe3O4nanoparticles,Fe3O4NPs)可用于实现对微囊的磁场驱动分离。该研究通过微胶囊膜内装载亲水四氧化三铁纳米粒(water soluble Fe3O4nanoparticles,W-Fe3O4NPs),同时使用荧光壳聚糖成膜,制备荧光可视顺磁性海藻酸–壳聚糖(alginate-chitosan,AC)微胶囊,用于中国仓鼠卵巢上皮细胞系(Chinese hamster ovary cell,CHO)细胞培养。结果表明,共培养细胞可以经荧光信号加以识别并利用磁场驱动加以分离。所构建的荧光可视顺磁性CHO细胞微胶囊在细胞微囊化共培养方面展现出较好的应用潜力。
Microencapsulation of cells is a valuable technique for inducing differentiation in cells, regulation of cell proliferation and increasing the production of cellular metabolites. Visual recognition and isolation of two microencapsulated cells play an important role in co-culture. As a magnetic medium, paramagnetic iron oxide nanoparticles (Fe3O4nanoparticles, Fe3O4NPs) can be used to achieve the magnetic field-driven separation of microcapsules. In this study, we prepared the fluorescent visual paramagnetic alginate-chitosan membrane by loading water-soluble Fe3O4nanoparticles (W-Fe3O4NPs) chitosan, AC) microcapsules for Chinese hamster ovary cell (CHO) cell culture. The results show that co-cultured cells can be identified by fluorescence signals and driven by magnetic field to be separated. The constructed fluorophorous paramagnetic CHO cell microcapsules showed good potential in microencapsulation of cells.