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以Atp6、ITS、Rpb2基因片段分别构建了蘑菇属中9个种[白蘑菇(Agaricus bisporus)、奶油蘑菇(棕色蘑菇)(A.bisporus)、(四孢)蘑菇(A.campestris)、大肥菇(A.bitorquis)、大紫蘑菇(A.auqustus)、野蘑菇(A.arvensis)、姬松茸(A.blazei)、酒色蘑菇(A.subrutilescens)、白林地蘑菇(A.silvicola)]20个菌株的分子遗传关系无根树。结果表明,ITS、Rpb2基因片段能够从某种程度对实验材料中的蘑菇种类进行区分;但Atp6基因片段因为进化较慢,其区分能力较差,不适于研究蘑菇属的遗传多样性。同时3个基因的联合分析较单一基因具有更好的分辨能力,所构建的亲缘关系树,可将9个种20个菌株划分为4个大的支系,进一步划分为7个明确的类群。
Nine species of Agaricus [Agaricus bisporus, A.bisporus, A. campestris, A. bitorquis, A. auquistus, A. arvensis, A. blazei, A. subrutii, A. silvicola 20 The strains of the molecular genetic relationship rootless tree. The results showed that the ITS and Rpb2 gene fragments could distinguish the mushroom species from the experimental materials to some extent. However, the Atp6 gene fragment was poorly differentiated because of its slow evolution and was not suitable for studying the genetic diversity of the genus Mushroom. At the same time, the joint analysis of three genes was better than single gene. The constructed phylogenetic tree could divide nine strains of 20 strains into four major genera and further divided them into seven distinct groups.