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纳米微晶纤维素(NCC)可由可再生资源制备,并且具有诸多特性,近年来成为研究热点。本文应用PFI磨对竹子溶解浆预处理,用纤维素酶水解制备纳米微晶纤维素,研究了酶解时间、酶解温度、酶用量对纳米微晶纤维素产率的影响,采用正交实验优化了工艺参数。并用高效液相色谱仪、马尔文激光粒度仪对水解液及NCC进行表征。结果表明:在酶用量2.736FPU/g、酶解时间3d、酶解温度50℃的条件下,纳米微晶纤维素的产率最高,达到19.13%;高效液相色谱分析表明水解液主要成分为葡萄糖,占总还原糖含量的71.06%,其次为纤维二糖12.39%,木糖7.68%;激光粒度分析表明NCC的Z均粒径为375.5nm。
Nano-microcrystalline cellulose (NCC) can be made from renewable resources, and has many characteristics, in recent years become a research focus. In this paper, PFI mill was used to pretreat bamboo dissolving pulp, cellulose nanocrystal cellulose was prepared by hydrolysis with cellulase. The effects of enzymolysis time, enzymolysis temperature and enzyme dosage on the yield of nanocrystalline cellulose were studied. Optimized process parameters. The hydrolyzate and NCC were characterized by high performance liquid chromatography and Malvern laser particle sizer. The results showed that the yield of nanocrystalline cellulose was the highest at the dosage of 2.736 FPU / g, the enzymolysis time of 3 d and the enzymolysis temperature of 50 ℃. The HPLC analysis showed that the main components of the hydrolyzate were Glucose, accounting for 71.06% of the total reducing sugar content, followed by cellobiose 12.39%, xylose 7.68%; laser particle size analysis showed that the average particle size of NCC was 375.5nm.