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采用三氟乙酸柱前衍生,利用高效液相色谱仪同时测定食品中的黄曲霉毒素B1、B2、G1、G2、M1、M2,样品经乙腈-水(体积比85∶15)提取、过滤后,以PuriToxSR多功能净化柱净化、浓缩,三氟乙酸(TFA)柱前衍生(1),C18色谱柱分离,荧光检测器检测,外标法定量。6种黄曲霉毒素经过衍生达到基线分离,标样线性良好,对添加黄曲霉毒素的牛奶、花生酱、饲料等样品进行加标回收,回收率在78%～102%,效果良好。
Pre-column derivatization with trifluoroacetic acid was used to determine aflatoxins B1, B2, G1, G2, M1 and M2 in food by high performance liquid chromatography. The samples were extracted with acetonitrile-water (85:15 by volume) Purified with PuriToxSR purification column, concentrated, TFA pre-column derivatization (1), C18 column separation, fluorescence detector detection, external standard method. The six aflatoxins were derived to baseline separation, and the standard samples were linear. The aflatoxin-added milk, peanut butter and other feed samples were spiked with a recovery of 78% -102%. The results were good.