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PCR技术是生物学研究上一项非常重要的技术,它是体外快速扩增特定基因或DNA序列的方法,只需在eppendorf离心管中建立反应,经数小时后,就能将极其微量的目的基因或某一特定DNA片段扩增数十万倍,甚至千百万倍.这项技术不仅可以用于获取和扩增目的基因,而且在癌症治疗的监控、临床医疗诊断以及法医学等众多领域都有着重要的用途,如此一项有重要作用的技术,笔者认为高中生必要理解和掌握这项技术.但是实际情况是学生对于PCR技术的掌握情况并不是很理想,对PCR技术原理和过程的掌握不够到位.究其原因,一方面由于PCR扩增DNA技术所需设备昂贵,一般中学很少具备动手操作的条件,另一方面PCR技术在高中教材中仅有少
PCR technology is a very important technology in biological research. It is a method for rapid amplification of specific genes or DNA sequences in vitro. The reaction can be established only in an eppendorf centrifuge tube, and after a few hours, a very small amount of purpose can be achieved Genes or a particular DNA fragment amplified hundreds of times, even millions of times.This technology can not only be used to obtain and amplify the purpose of the gene, but also in cancer treatment monitoring, clinical diagnosis and forensic medicine and many other areas Has an important use, such an important role in technology, I believe that high school students need to understand and master the technology.But the actual situation is that students grasp the situation of PCR technology is not very satisfactory, the principle of PCR technology and process control Not enough.Investigation of the reasons, on the one hand due to the expensive equipment required for PCR amplification of DNA technology, generally have very few hands-on conditions in high school, on the other hand PCR technology in high school textbooks only less