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[目的]评价大鼠妊娠早期接触丙烯腈对子代成年后心肌缺血再灌注损伤敏感度的影响。[方法]2月龄雄性大鼠5只和雌性大鼠12只随机分组,对照组及丙烯腈低、中、高浓度组,合笼,确认雌性大鼠怀孕后于孕期第1～7天给予不同浓度丙烯腈染毒,仔鼠正常条件下饲养至3个月作为实验动物;使用Langendorff系统分别记录心脏复跳后和缺血60min再灌注后各组仔鼠心脏心率(heart rate,HR)、冠脉流量(coronary flow,CF)、左室舒张末压(left ventricular end-diastolic pressure,LVEDP)、左室收缩压(left ventricular systolic pressure,LVSP)、最大压力导数[(dp/dt)_(max)]和最小压力导数[(dp/dt)_(min)],计算梗死心肌占总心脏重量的比例。[结果]对照组和丙烯腈染毒组心脏复跳后基础心功能、冠脉流量、心率差异无统计学意义(P>0.05);但缺血再灌注后丙烯腈染毒组上述指标明显低于对照组(P<0.05,P<0.01),而且中浓度丙烯腈染毒组上述指标低于低浓度染毒组(P<0.05,P<0.01)。丙烯腈染毒组梗死心肌重量占总心脏重量比例明显大于对照组(P<0.05)。[结论]大鼠妊娠早期接触丙烯腈对子代成年后心肌缺血再灌注损伤敏感度增加;而且接触丙烯腈较高浓度者其损伤敏感度亦增加,诱发心律失常比例升高。
[Objective] To evaluate the influence of early pregnancy exposure to acrylonitrile on myocardial ischemia-reperfusion injury in offspring adult. [Methods] Five male and two female rats at 2 months old were randomly divided into control group and low, medium and high concentrations of acrylonitrile group. After being caged, it was confirmed that female rats were given 1 to 7 days after pregnancy The animals were exposed to acrylonitrile at different concentrations for three months under normal conditions as experimental animals. The Langendorff system was used to record heart rate (HR), heart rate The coronary flow (CF), left ventricular end-diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP) and maximum pressure derivative [(dp / dt) max)] and the minimum pressure derivative [(dp / dt) _ (min)], the ratio of infarcted myocardium to total cardiac weight was calculated. [Results] The baseline heart function, coronary flow and heart rate in control group and acrylonitrile-treated group were not statistically significant (P> 0.05) after cardiopulmonary resuscitation. However, the indexes of acrylonitrile-treated group were significantly lower In the control group (P <0.05, P <0.01), and the above indexes in the medium concentration acrylonitrile group were lower than those in the low concentration group (P <0.05, P <0.01). The ratio of myocardial infarction weight to total heart weight in the acrylonitrile group was significantly larger than that in the control group (P <0.05). [Conclusion] Exposure to acrylonitrile in early pregnancy increased the susceptibility to myocardial ischemia-reperfusion injury in adult offspring. Exposure to higher concentrations of acrylonitrile increased the susceptibility to injury and increased the proportion of arrhythmia induced.