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目的探讨内毒素(即脂多糖,lipopolysaccharide,LPS)刺激前后 Toll 样受体4(Tolllike receptor 4,TLR4)mRNA 和核因子 kB(nuclear factor kB,NF-kB)p50亚型 mRNA 在正常鼻黏膜上皮细胞中的表达及意义。方法在15例成人鼻中隔偏曲患者(无鼻-鼻窦炎)矫正术中,取正常中鼻甲外侧黏膜组织各2份行组织外植块培养,其中15份加 LPS 刺激培养为 LPS 组,另外为培养组。应用 HE 染色光镜下观察鼻黏膜组织上皮细胞的病理形态学改变;应用核酸分子原位杂交技术检测离体培养正常鼻黏膜组织上皮细胞中 TLR4 mRNA 和 NF-kB p50 mRNA 的表达情况。结果①LPS刺激后,光镜下见正常鼻黏膜上皮细胞纤毛有粘连成束、胞体增大的病理形态学改变;②LPS 组 TLR4mRNA 表达明显高于培养组;LPS 组表达阳性区平均吸光度为1.283±0.027,培养组为0.538±0.038,二组间差异有统计学意义(t=1.761,P<0.05);③NF-kB p50 mRNA 在 LPS 组均出现阳性表达,而培养组阳性率仅为26.7%。LPS 组明显高表达于培养组,且以胞核表达为主,LPS 组表达阳性区平均吸光度为1.668±0.037;培养组为0.372±0.052,二组间差异有统计学意义(t=2.624,P<0.01)。结论 LPS 能通过 TLR4活化 NF-kB p50,进而激活正常鼻黏膜上皮细胞。这可能在 LPS 对鼻黏膜上皮细胞激活和损伤效应中具有一定的作用。
Objective To investigate the expression of Toll-like receptor 4 (TLR4) mRNA and NF-κB p50 subtype mRNA in normal nasal epithelium before and after stimulation with lipopolysaccharide (LPS) Cell expression and significance. Methods In 15 cases of adult nasal septum deviation patients (no nasosinusitis) correction surgery, take the normal middle turbinate 2 cases of lateral mucosal tissue explant culture, of which 15 were supplemented with LPS stimulated culture LPS group, the other for Cultivation group. Pathological changes of nasal mucosa epithelial cells were observed under HE staining. The expression of TLR4 mRNA and NF-κB p50 mRNA in normal nasal mucosa epithelial cells was detected by in situ hybridization. Results ① After LPS stimulation, the histopathological changes of ciliated mucosa of normal nasal mucosa epithelial cells were observed under light microscope. The expression of TLR4 mRNA in LPS group was significantly higher than that in culture group. The average absorbance of LPS group was 1.283 ± 0.027 (T = 1.761, P <0.05). ③NF-kB p50 mRNA expression in LPS group was positive, while the positive rate of culture group was only 26.7%. LPS group was significantly higher expression in the culture group, and the main nuclear expression, LPS group positive expression zone average absorbance was 1.668 ± 0.037; culture group was 0.372 ± 0.052, the difference between the two groups was statistically significant (t = 2.624, P <0.01). Conclusion LPS can activate NF-κB p50 by TLR4 and activate normal nasal epithelial cells. This may have a role in the activation and injury of nasal mucosal epithelial cells by LPS.