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目的:了解2011年乌鲁木齐地区腹泻患儿腺病毒(AdV)的感染情况,分析该地区AdV的基因亚型,进行AdV感染分子流行病学调查。方法:采集在新疆维吾尔自治区人民医院住院和门诊≤5岁腹泻患儿粪便标本315份,采用胶体金法检测轮状病毒(RV)抗原,采用聚合酶链反应(PCR)法对上述标本进行AdV检测,并对AdV阳性标本测序明确基因型。结果:315份粪便标本中共检出AdV阳性12例,总阳性率为3.81%,低于RV检出率26.35%,AdV和RV混合感染3例。AdV阳性标本中肠道腺病毒(EAdV,F组)Ad41占2.54%(8/315),其次是非肠道腺病毒(NEAdV)占1.27%(4/315),分别为Ad1、Ad3、Ad5、Ad7、Ad31型。12份阳性标本测序结果提示该地区AdV与参考株的核苷酸序列同源性为73%～99%。结论:乌鲁木齐地区AdV感染呈全年散发流行,未表现出明显的季节性,其主要的流行株为Ad41型。
Objective: To understand the infection of adenovirus (AdV) in children with diarrhea in Urumqi in 2011, analyze the genotypes of AdV in this area and investigate the molecular epidemiology of AdV infection. Methods: A total of 315 stool samples of children with diarrhea who were hospitalized and outpatient ≤5 years old were collected from the People’s Hospital of Xinjiang Uygur Autonomous Region. The antigen of rotavirus (RV) was detected by colloidal gold assay. The samples were subjected to polymerase chain reaction (PCR) Detection, and AdV positive specimens sequenced clear genotypes. Results: Of the 315 stool samples, 12 were positive for AdV, the overall positive rate was 3.81%, lower than the detection rate of RV by 26.35%, and 3 cases were mixed with AdV and RV. Ad41 positive samples were 2.54% (8/315) for Ad41 and 1.27% (4/315) for non-enteric adenovirus (AdAd, Ad3, Ad5, Ad7, Ad31 type. Sequencing results of 12 positive specimens suggested that nucleotide sequence homology between AdV and reference strain was 73% -99% in this region. Conclusion: The infection of AdV in Urumqi region was epidemic throughout the year, showing no obvious seasonality. The main epidemic strain was Ad41.