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目的研究赤芝子实体石油醚部位的化学成分,寻找具有抗肿瘤活性的先导化合物。方法采用硅胶、Sephadex LH-20等柱色谱以及半制备高效液相色谱等手段分离纯化,并通过理化性质和波谱数据鉴定化合物的结构;采用MTT法测试分离化合物对人宫颈癌细胞的细胞毒活性。结果从赤芝子实体石油醚部位分离得到9个化合物,分别鉴定为3,7-二羟基-12-乙酰氧基-11,15,23-三羰基羊毛甾-8,22-二烯-26-酸(12-acetoxy-3,7-dihydroxy-11,15,23-trioxolanost-8,22-dien-26-oic acid,1)、灵芝三醇M(ganoderitriol M,2)、亚油酸乙酯[(10E,13E)-ethyl octadeca-10,13-dienoate,3]、亚油酸[(10E,13E)-octadeca-10,13-dienoic acid,4]、二十四碳酸(tetracosanoic acid,5)、3β-羟基-5α,8α-过氧麦角甾-6,9,22-三烯(3β-hydroxy-5α,8α-epidioxyergosta-6,9,22-triene,6)、麦角甾-7,22-二烯-3-酮(ergosta-7,22-dien-3-one,7)、过氧麦角甾醇(ergosterol peroxide,8)和麦角甾醇(ergosterol,9)。结论化合物1的波谱数据为首次报道,化合物3、4、7为首次从赤芝中分离得到。化合物2显示出中等强度的癌细胞抑制活性,其IC50为12μmol·L-1。
Objective To study the chemical constituents of the petroleum ether part of Ganoderma lucidum fruiting bodies and to find the lead compounds with antitumor activity. Methods The compounds were separated and purified by column chromatography on silica gel, Sephadex LH-20 and semi-preparative high performance liquid chromatography (HPLC). The structures of the compounds were identified by physicochemical properties and spectral data. The cytotoxic activity of the isolated compounds on human cervical cancer cells . Results Nine compounds were isolated from petroleum ether fractions of Ganoderma lucidum fruiting bodies and identified as 3,7-dihydroxy-12-acetoxy-11,15,23-tricarbonyl lanosterol-8,22-diene-26- (12-acetoxy-3,7-dihydroxy-11,15,23-trioxolanost-8,22-dien-26-oic acid, 1), ganoderitriol M (2), ethyl linoleate [(10E, 13E) -ethyl octadeca-10,13-dienoate, 3], linoleic acid [(10E, 13E) -octadeca-10,13-dienoic acid, 4], tetracosanoic acid ), 3β-hydroxy-5α, 8α-hydroxy-5α, 8α-epidioxyergosta-6,9,22-triene, 6, (Ergosta-7,22-dien-3-one, 7), ergosterol peroxide (8) and ergosterol (9). Conclusion The spectral data of compound 1 is reported for the first time. Compounds 3, 4, and 7 were isolated from Ganoderma lucidum for the first time. Compound 2 showed moderate-intensity cancer cell inhibitory activity with an IC50 of 12 μmol·L-1.