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目的:探讨抗中性粒细胞胞浆抗体(ANCA)在活动期系统性红斑狼疮(SLE)患者中临床意义。方法:通过间接免疫荧光法(IIF)及酶联免疫吸附分析法(ELISA)检测63例处于活动期的SLE的血清ANCA,并分析ANCA与SLE临床表现及其他实验室检查结果之间的关系。结果:用IIF方法检测时ANCA在SLE的阳性率25.4%,全部是核周型AN-CA(p-ANCA)。用ELISA方法检测靶抗原,乳铁蛋白Lactofertin(LF)为主要的靶抗原。ANCA在SLE合并皮肤血管炎或肾脏受累、病情活动及抗ds-DNA抗体阳性时的阳性率显著高于相应对照组。结论:ANCA可能参与了SLE血管炎的发病过程。
Objective: To investigate the clinical significance of anti-neutrophil cytoplasmic antibody (ANCA) in patients with active systemic lupus erythematosus (SLE). Methods: Serum ANCA was measured in 63 active SLE patients by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA), and the relationship between ANCA and SLE clinical manifestations and other laboratory findings was analyzed. Results: The positive rate of ANCA in SLE was 25.4% when tested by the IIF method, all of which were perinuclear AN-CA (p-ANCA). The target antigen was detected by ELISA, Lactofertin (LF) was the main target antigen. The positive rate of ANCA in SLE combined with skin vasculitis or kidney involvement, disease activity and anti-dsDNA antibody was significantly higher than that of corresponding control group. Conclusion: ANCA may be involved in the pathogenesis of SLE vasculitis.