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尽管发描电镜样品制备较透射电镜样品制备简单，但在样品制备过程中须用一些特殊的仪器，如临界点干燥仪、离子镀膜仪等。鉴于近年来微波辐射术在扫描电镜样品制备中的应用，本文采用微波辐射术对六胺银染色的扫描电镜样品（大鼠气管及十二指肠）进行了初步观察，取得了良好的结果。微波辐射是用SMC“一70E－2型”微波炉（广东顺德蚬华微波制品厂），输出功率650W，微波频率2450MHZ，火力档10档（PI－10）可调。样品经微波辐射固定（3％戊二醒）、后固定（1％OsO。）后，入l％过碘酸，辐射30秒，火力档PI；六胺银染色，辐射1分钟，火力档PZ。六胺银染液的配方：15％六次甲基四胺4ml，5％硼砂Zml，4％硼酸07ml，双蒸水25ml，染色前加入10％硝酸银lml，混匀。丙酮梯度脱水后，将样品置于微波炉内干燥，辐射2分钟，火力档P3，不需金属镀膜，即可在AMR－1000B型扫描电镜下观察。六胺银染色是通过过碘酸的氧化作用，将组织中的羧基氧化成醛基，而醛基能使六胺银还原成金属银，它均匀地沉积在含有凌基组织样品的表面，形成连续而均匀的金属薄膜，使样品获得良好的导电性能。微波干燥使经丙酮脱水的样品被整体加热，丙酮在组织内被迅速气化，使样品的干燥变得简单和快捷。本方法简便、快速，在制样过程无需特殊
Although the preparation of SEM is simpler than that of TEM, some special instruments such as critical point dryer and ion plating instrument are needed during sample preparation. In view of the application of microwave radiography in the preparation of SEM in recent years, microwave radiation was used in the preliminary observation of the silver-stained SEM (rat trachea and duodenum) with good results. Microwave radiation is using SMC “a 70E-2” microwave oven (Guangdong Shunde Hua China Microwave Products Factory), the output power of 650W, microwave frequency 2450MHZ, fire stalls 10 files (PI-10) adjustable. The sample was fixed by microwave (3% pentylene waken), fixed after (1% OsO.), Into l% periodic acid, radiation 30 seconds, PI fire; silver amine staining, radiation 1 minute, fire file PZ . Hexamine silver dye solution: 15% hexamethylenetetramine 4ml, 5% borax Zml, 4% boric acid 07ml, double distilled water 25ml, before adding 10% silver nitrate lml, and mix. Acetone gradient dehydration, the sample was placed in a microwave oven to dry, radiation 2 minutes, fire P3, without metal coating, you can observe in the AMR-1000B scanning electron microscope. Hexamine silver staining is the oxidation of the periodic acid oxidation of the carboxyl groups in the tissue into aldehyde groups while aldehyde groups reduce the hexamine silver to metallic silver which is uniformly deposited on the surface of the sample containing Lingji tissue to form Continuous and uniform metal film, the sample to obtain good electrical conductivity. The microwave drying heats the sample dehydrated in acetone as a whole, and the acetone is rapidly vaporized in the tissue to make the drying of the sample easier and faster. This method is simple, fast, no special sample preparation process