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目的 :分析KM等3个封闭群小鼠线粒体DNA(mtDNA)的多态性 ,探讨实验小鼠的遗传监测方法。方法 :PCR RFLP技术 ,即PCR技术结合限制性内切酶片段长度多态分析(Restrictionfragmentlengthpolymorphism,RFLP)。结果 :mtDNAD Loop、tRNA1基因、ND3基因片段经HaeⅢ、HinfⅠ、EcoRV、HindⅢ、HpaⅠ、BamHⅠ、ApaⅠ、NdeⅡ、XhoⅠ、XbaⅠ、AluⅠ、RsaⅠ、StuⅠ、DraⅠ、AvaⅠ、HaeⅡ16种内切酶分别消化后 ,KM、NIH、ICR等小鼠品系内、品系间均表现出相同的酶切图谱 ,且与近交系小鼠BALB/C完全相同 ,未发现多态性。结论 :KM、NIH、ICR小鼠与绝大多数实验小鼠一样 ,遗传背景较为狭窄 ,不同品系小鼠间遗传背景的差异远远低于动物种属间的差异。
OBJECTIVE: To analyze the polymorphisms of mitochondrial DNA (mtDNA) in three closed mice such as KM and to explore the genetic monitoring methods in experimental mice. Methods: PCR RFLP technique, ie PCR technique combined with restriction fragment length polymorphism (RFLP). Results: The mtDNAD Loop, tRNA1 gene and ND3 gene fragment were digested with 16 kinds of endonucleases of HaeⅢ, HinfⅠ, EcoRV, HindⅢ, HpaⅠ, BamHⅠ, ApaⅠ, NdeⅡ, XhoⅠ, XbaⅠ, AluⅠ, RsaⅠ, StuⅠ, DraⅠ, , KM, NIH, ICR and other mouse strains showed the same restriction map, and the same inbred mice BALB / C, no polymorphism was found. Conclusion: KM, NIH and ICR mice, like most experimental mice, have a relatively narrow genetic background. The genetic background differences among mice of different strains are much lower than those of animal species.