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[目的]探讨不同基因型水稻的抗性机理。[方法]通过盆栽试验研究了有机酸和EDTA对镉污染土壤上水稻生理生化指标的影响。[结果]单独和混合施加有机酸和EDTA均显著提高了镉污染土壤上水稻的SOD活性,其POD、CAT活性和MDA含量均比镉污染对照显著降低。添加有机酸和EDTA分别使低镉污染土壤中秀水63 MDA含量比低镉对照降低了7.5%和16.4%。加入有机酸和ED-TA后,水稻体内游离脯氨酸含量高于镉污染对照且相同处理下Ⅱ优527体内的游离脯氨酸含量高于秀水63。秀水63的根系活力的变化比Ⅱ优527小。在低镉污染处理中,有机酸和EDTA的添加降低了水稻的根系活力;在高镉污染处理中,仅有机酸的添加显著提高了水稻的根系活力。[结论]有机酸和EDTA能显著降低水稻POD和CAT活性及MDA含量,并增加SOD活性。
[Objective] The research aimed to study the resistance mechanism of different genotypes of rice. [Method] The effects of organic acids and EDTA on the physiological and biochemical indexes of rice on Cd polluted soil were studied through pot experiment. [Result] The application of organic acids and EDTA alone and in combination significantly increased the SOD activity of rice plants on Cd-contaminated soil, and their POD, CAT activities and MDA contents were significantly lower than those of Cd-contaminated control. The addition of organic acids and EDTA decreased the MDA content of Xiushui 63 in low Cd-contaminated soil by 7.5% and 16.4%, respectively, compared with the low-Cd control. After addition of organic acids and ED-TA, the content of free proline in rice was higher than that of cadmium, and the content of free proline in Ⅱyou 527 was higher than that in Xiushui 63 under the same treatment. Xiushui 63 root activity changes than Ⅱ You 527 small. In the treatment of low cadmium pollution, the addition of organic acids and EDTA reduced the root activity of rice. In the treatment of high cadmium pollution, only the addition of organic acids significantly increased the root activity of rice. [Conclusion] Organic acids and EDTA could significantly reduce POD and CAT activity and MDA content and increase SOD activity in rice.