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目的 :探讨肿瘤坏死因子α(TNFα,4× 10 5U/L)对血管内皮细胞增殖的影响。方法 :将传代培养的脐静脉内皮细胞随机分为实验组和对照组两组 ,分别在细胞传代时加入含TNFα(TNFα含量为 4× 10 5U/L)和等量的不含TNFα的培养液 ,通过描绘生长曲线及运用图像分析测定DNA相对含量来观察两组细胞增殖情况。结果 :处理因素作用 3天内实验组细胞增殖快于对照组 ,而 3天后则慢于对照组。结论 :TNFα(4× 10 5U/L)对培养的脐静脉内皮细胞的增殖的影响具有双向性。
Objective: To investigate the effect of tumor necrosis factor α (TNFα, 4 × 10 5 U / L) on the proliferation of vascular endothelial cells. Methods: The subcultured umbilical vein endothelial cells were randomly divided into experimental group and control group. TNFα (TNFα content was 4 × 10 5 U / L) and equal amount of culture medium without TNFα , By depicting the growth curve and the use of image analysis to determine the relative content of DNA to observe the proliferation of two groups of cells. Results: The effect of treatment factors within 3 days in experimental group cell proliferation faster than the control group, but after 3 days was slower than the control group. CONCLUSION: The effect of TNFα (4 × 10 5 U / L) on the proliferation of cultured human umbilical vein endothelial cells is bi-directional.