论文部分内容阅读
目的:探讨和厚朴酚对鼻咽癌细胞株CNE-1的增殖、迁移与侵袭能力的影响,并研究其初步机制。方法:采用MTT法检测不同浓度的和厚朴酚(0、20、40、80与160μmol/L)对CNE-1细胞存活率的影响;采用划痕实验和Transwell小室检测细胞的迁移和侵袭能力;采用Western Blot检测PTEN、p-Akt与p-mTOR蛋白表达水平。结果:MTT法检测结果显示,和厚朴酚呈浓度依赖性抑制Eca-109细胞的生长,当浓度到达80μmol/L时,抑制作用达到最大;和厚朴酚干预后,CNE-1细胞迁移和侵袭能力显著下降,PTEN蛋白表达水平显著上调,p-Akt与p-mTOR蛋白表达水平显著下降。结论:和厚朴酚具有抑制鼻咽癌细胞株CNE-1增殖、迁移和侵袭的作用,其作用机制可能与和厚朴酚抑制PTEN/PI3k/Akt信号通路有关。
Objective: To investigate the effects of honokiol on the proliferation, migration and invasion of nasopharyngeal carcinoma cell line CNE-1 and to investigate its primary mechanism. Methods: The effects of different concentrations of honokiol (0, 20, 40, 80 and 160 μmol / L) on the survival rate of CNE-1 cells were detected by MTT assay. Scratch and Transwell chambers were used to detect the cell migration and invasion The protein expression of PTEN, p-Akt and p-mTOR was detected by Western Blot. Results: The results of MTT assay showed that honokiol inhibited the growth of Eca-109 cells in a concentration-dependent manner. When the concentration reached 80μmol / L, the inhibitory effect reached the maximum. After honokiol intervention, CNE-1 cell migration and Invasive ability was significantly decreased, PTEN protein expression was significantly increased, p-Akt and p-mTOR protein expression decreased significantly. Conclusions: Honokiol can inhibit the proliferation, migration and invasion of nasopharyngeal carcinoma cell line CNE-1, which may be related to the inhibition of PTEN / PI3k / Akt signaling pathway by honokiol.