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目的应用GFS 24Y试剂盒,对中国广东汉族和新疆维吾尔族共584个无关男性样本进行24个Y-STR基因座分型,统计其基因座、单倍型的遗传多态性,并分析其群体差异。方法采用磁珠法提取样本DNA后用GFS24Y试剂盒扩增,3130XL遗传分析仪进行基因分型,根据分型结果进行统计分析。结果获得了中国广东汉族与新疆维吾尔族共584个样本24个Y-STR基因座的分型结果及各等位基因频率分布资料。在广东汉族群体中,各基因座的基因多样性(GD值)为0.560 6~0.963 9,由24个基因座组成的单倍型共有299个,单倍型多样性(HD值)为0.999 93;在新疆维吾尔族中,GD值为0.443 9~0.957 9,共观察到282个单倍型,HD值为0.999 85。两个民族未观察到共有单倍型。通过两个群体间的比较,共有22个Y-STR基因座等位基因频率分布在两个民族之间存在差异(P<0.05),各基因座在两个民族的遗传分化系数(Fst值)为0.000 767 9(DYS481)~0.247 946(DYS520)。结论 24个Y-STR基因座在中国广东汉族与新疆维吾尔族人群中均有较高的多态性,并显示出明显的民族特征,在法医亲权鉴定和个体识别、群体遗传学等领域均有重要的应用价值。
A total of 24 Y-STR loci were genotyped in 584 unrelated male samples of Han nationality in Guangdong province and Uygur ethnic population in Xinjiang using GFS 24Y kit. The genetic polymorphisms of loci and haplotypes were analyzed and their populations difference. Methods The sample DNA was extracted by magnetic beads method and then amplified by GFS24Y kit. Genotyping was performed by 3130XL genetic analyzer, and statistical analysis was carried out according to typing results. Results The genotyping results of 24 Y-STR loci from 584 samples of Han and Xinjiang in China and the frequency distribution of alleles were obtained. In Guangdong Han population, the genetic diversity (GD) of each locus ranged from 0.560 6 to 0.963 9. There were 299 haplotypes from 24 loci, and the haplotype diversity (HD) was 0.999 93 In Uygur Xinjiang, GD values ranged from 0.443 9 to 0.957 9, a total of 282 haplotypes were observed with an HD value of 0.999 85. No common haplotype was observed between the two ethnic groups. A total of 22 Y-STR loci allele frequency distributions differed between the two ethnic groups (P <0.05) by comparing the two populations. The genetic differentiation coefficient (Fst) 0.000 767 9 (DYS481) to 0.247 946 (DYS520). Conclusion The 24 Y-STR loci are highly polymorphic in Han population of Guangdong province and Uyghur population of Xinjiang province, and show obvious ethnic characteristics. They are all identified in the fields of forensic paternity testing, individual identification and population genetics Have important application value.