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血清学方法在研究黄化病毒组方面一直是特别有用的,因为这类病毒不能用机械方法传播到植物上,病毒在病株中的含量又很低,在用蚜虫作媒介进行病毒传播研究中,会遇到各种各样的困难.目前,有用的技术已从一种侵染性中和法延伸到酶联免疫吸附测定(ELISA).尽管这些方法有许多优点,但是由于在制备动物抗血清中,要准备足够数量的纯化病毒有某些困难;同时,由于黄化病毒之间的血清学特异性,以及不同动物,甚至同一动物不同个体的抗血清之间,常常发生变异,因此需要多种多样的抗血清,而目前这种抗血清的供应仍很有限;所以这些方法在应用上还是受限制的.
Serological methods have been of particular utility in the study of the yellow virus group because such viruses can not be mechanically transmitted to plants and the virus levels in the diseased plants are very low. In aphid-borne virus-borne studies , There are a variety of difficulties encountered Currently, useful techniques have been extended from an invasive neutralization assay to an enzyme-linked immunosorbent assay (ELISA) Although these methods have many advantages, In serum, there are some difficulties in preparing a sufficient number of purified viruses; at the same time, due to the serological specificity between the avian viruses and the variation of the antisera in different animals and even in different animals of the same animal, it is necessary A wide variety of antisera are available and the current availability of such antisera is still limited; therefore, these methods are still limited in their application.