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目的建立固相萃取-高效液相色谱法测定食品中2-(4-甲氧基苯氧基)-丙酸钠的分析方法。方法样品采用氨水溶液(1∶9,V/V)溶解后固相萃取净化,在Kromasil C18色谱柱(250 mm×4.6 mm×5μm)上,以甲醇/磷酸溶液(1∶1,V/V,p H=3.0)为流动相进行分离,紫外224 nm波长检测,外标法定量。结果 2-(4-甲氧基苯氧基)-丙酸钠在0.50 mg/L~50.0 mg/L范围内具有良好的线性关系(相关系数r=0.9995),回收率为87.4%~99.0%,RSD<5.0%,定量检出限为1.0 mg/kg。结论该方法具有前处理简单、重现性好的优点,能满足实际工作的需要。
Objective To establish a method for the determination of 2- (4-methoxyphenoxy) -propionate in food by solid-phase extraction and high performance liquid chromatography. Methods The samples were dissolved in aqueous ammonia solution (1: 9, V / V) and purified by solid-phase extraction. The residue was purified on a Kromasil C18 column (250 mm × 4.6 mm × 5 μm) , p H = 3.0) as the mobile phase separation, UV detection wavelength of 224 nm, external standard method. Results The linear range of 2- (4 - methoxyphenoxy) - propionate was 0.50 mg / L ~ 50.0 mg / L with a correlation coefficient of 0.9995 and the recoveries were 87.4% ~ 99.0% , RSD <5.0%, and the limit of quantification was 1.0 mg / kg. Conclusion The method has the advantages of simple preprocessing and good reproducibility, and can meet the needs of practical work.