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应用经羟基磷灰石纯化，偶联长链生物素ＢＡＣＨ（Ｂｉｏｔｉｎａｍｉｎｏｃａｐｒｙｌｈｙｄｒａｚｉｄｅ）的两株主要识别卵相关不同表位的单克隆抗体建立检测灵敏度达微微克水平的硷性磷酸酶抗原捕获酶联试验（ＢＡ－ＡＰＥＬＩＳＡ），对粗制日本血吸虫卵抗原ＳＥＡｊ－ＴＣＡ的检测极限，２Ｈ１０－ＥＬＩＳＡ可达１—０．３ｎｇ／ｍｌ，仅有效地检出日本血吸虫种特异的卵抗原表位分子；而２Ｈ１－ＥＬＩＳＡ则达３．２ｎｇ／ｍｌ，亦能灵敏地检示曼氏血吸虫的卵抗原组分。两组试验反复试用于日本血吸虫病例及正常人群血样检测，显示有非常明显的ＯＤ消光读数差异。同时采用已建立的１Ｂ１０－ＡＰＥＬＩＳＡ检测肠相关ＣＡＡ系列，以平行检测的健康对照组消光ＯＤ均值＋３ＳＤ为阳性界值，对３组采自不同流行区的慢性或混合型病例同批血样进行肠相关ＣＡＡ及卵相关２Ｈ１０和２Ｈ１的二联或三联叠加检测，其累计检出率都得到不同程度的提高，尤以低度流行区的慢性病例组更为明显。
Application of two monoclonal antibodies recognizing different egg-related epitopes purified by hydroxyapatite and coupled to BACH (Biotin aminocaprylhydrazide) Alkaline phosphatase antigen capture enzyme-linked assay for detection of pico- BA-APELISA), the limit of detection of the crude antigen of SEAj-TCA of Schistosoma japonicum was 2H1-ELISA up to 1-0.3ng / ml, which was only effective for the detection of Schistosoma japonicum specific egg antigen epitopes. -ISA was up to 3.2ng / ml, but also sensitive detection of Schistosoma mansoni egg antigen component. Two groups of tests were repeatedly tested in blood samples of Schistosoma japonicum cases and normal population, showing a very significant OD extinction reading difference. At the same time, the established 1B10-AP ELISA was used to detect the intestinal-related CAA series. The OD value + 3SD of extinction in the parallel control group was positive cut-off value. The three groups of chronic or mixed cases collected from different epidemic areas were enrolled CAA and egg-related 2H10 and 2H1 double or triple superposition detection, the cumulative detection rate has been increased to varying degrees, especially in the low prevalence of chronic cases more obvious.