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对正常人晶体及白内障晶体上皮细胞进行体外培养,并观察体外培养的晶体上皮细胞的生物学特性及组织学变化。最后应用5-氟脲嘧啶、高三尖杉酯碱及骆驼蓬碱对组织培养的晶体上皮细胞进行药物抑制实验。结果显示,人类晶体上皮细胞在体外生存能力有限,体外的生长能力与供体年龄呈负相关。高三尖杉酯碱及骆驼蓬碱可有效地抑制晶体上皮细胞的生长繁殖。根据对培养的晶体上皮细胞生长特性的观察,提出使用边缘光滑的撕囊术可能会降低白内障术后后囊混浊的发生率。
The normal human lens and cataract lens epithelial cells cultured in vitro and observed in vitro cultured epithelial cells of the biological characteristics and histological changes. Finally, 5-fluorouracil, homoharringtonine and harman alkali were used to inhibit the growth of cultured human epithelial cells. The results showed that human crystal epithelial cells in vitro survival ability is limited, in vitro growth capacity and donor age was negatively correlated. Homoharringtonine and harman alkali can effectively inhibit the growth and reproduction of the lens epithelial cells. Based on the observation of the growth characteristics of cultured lens epithelial cells, it was suggested that the use of smooth-edged capsulotomy may reduce the incidence of posterior capsular opacification after cataract surgery.