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用ＰＣＲ方法从鲑精ＤＮＡ中分离出编码蛙鱼降钙素（ｓＣＴ）基因，并将其插入带有谷脱甘肽转移酶（ＧＳＴ）基因的融合表达载体ｐＧＥＸ－２Ｔ上，转入大肠杆菌ＪＭ１０９中，ＩＰＴＧ诱导表达。融合蛋白（ＧＳＴ－ｓＣＴ）表达量占菌体总蛋白量的３８％～４０％。用亲和层析方法纯化该融合蛋白，达８０％电泳纯，降钙素酶联免疫试验呈阳性反应。这为生产重组ｓＣＴ进而研究它的功能和应用打下了基础。
The gene encoding frogfish calcitonin (sCT) was isolated from salmon sperm DNA by PCR and inserted into the fusion expression vector pGEX-2T with the glutathione S-transferase (GST) gene and transformed into Escherichia coli In JM109, IPTG induced expression. The fusion protein (GST-sCT) expression amount accounted for 38% to 40% of total bacterial protein. The fusion protein was purified by affinity chromatography and reached to 80% electrophoresis purity. The calcitonin enzyme-linked immunosorbent assay showed a positive reaction. This laid the foundation for the production of recombinant sCTs and their function and application.