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目的:研究胃癌组织中RASAL1基因启动子的甲基化状态并分析其与临床病理资料的关系,初步探讨RASAL1基因启动子甲基化在胃癌发生发展中的意义。方法:取40例胃癌患者手术标本,使用甲基化特异性PCR(MSP)技术分别检测胃癌组织及相应癌旁组织中RASAL1基因启动子的甲基化状态,并分析其与胃癌临床病理特征的关系。结果:胃癌组织和癌旁组织中RASAL1基因启动子甲基化率分别为70%(28/40)和30%(12/40),胃癌组织中RASAL1基因启动子的甲基化发生率明显高于癌旁组织,P<0.01。胃癌组织中RASAL1基因启动子的甲基化率与性别(P=0.622)、年龄(P=0.168)无明显相关;但与分化程度(P=0.006)、肿瘤大小(P=0.043)、侵袭深度(P=0.015)和淋巴结转移相关(P=0.010);在肿瘤体积大、分化程度差、侵袭深度较深和有淋巴结转移的胃癌组织中,RASAL1基因启动子甲基化率明显增高。结论:胃癌组织中RASAL1基因启动子区域甲基化发生率高,且与分化程度及进展阶段相关,提示RASAL1基因启动子的异常甲基化可能参与了胃癌的发生发展过程。
OBJECTIVE: To study the methylation status of RASAL1 gene promoter in gastric cancer and analyze its relationship with clinicopathological data, and to explore the significance of RASAL1 promoter methylation in the development of gastric cancer. Methods: The methylation status of RASAL1 gene promoter in gastric cancer tissue and corresponding paracancerous tissues was detected by methylation-specific PCR (MSP) and its correlation with the clinicopathological characteristics of gastric cancer relationship. Results: The promoter methylation rates of RASAL1 gene in gastric cancer tissues and adjacent tissues were 70% (28/40) and 30% (12/40), respectively. The methylation of RASAL1 gene promoter in gastric cancer tissues was significantly higher In paracancerous tissues, P <0.01. The methylation rate of RASAL1 promoter in gastric cancer tissues was not significantly correlated with gender (P = 0.622) and age (P = 0.168), but not with the degree of differentiation (P = 0.006) (P = 0.015) and lymph node metastasis (P = 0.010). The methylation rate of RASAL1 promoter in gastric cancer tissues with large volume, poor differentiation, deep invasion and lymph node metastasis was significantly increased. Conclusion: The incidence of methylation of RASAL1 promoter region in gastric cancer is high, which is related to the differentiation stage and progression stage. It suggests that abnormal methylation of RASAL1 promoter may be involved in the development and progression of gastric cancer.