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目的研究增殖细胞核抗原（ＰＣＮＡ）在胃良、恶性肿瘤中的表达及其临床意义。方法应用免疫组化技术（ＡＢＣ法），对胃肿瘤组织切片（胃腺瘤性息肉１０例，胃癌９４例）中ＰＣＮＡ含量进行半定量检测，观察其与胃肿瘤临床病理参数及预后的关系。结果（１）胃粘膜不同病理状态下ＰＣＮＡ指数差异显著（各组间Ｐ值均小于０．０１）；（２）ＰＣＮＡ指数同患者的性别和年龄不相关（Ｐ＞０．０５），而与肿瘤的生长方式、组织类型、浆膜层受侵与否、淋巴结转移与否和临床分期关系密切（Ｐ值均小于０．０５）；（３）ＰＣＮＡ指数与胃癌的预后密切相关（Ｐ＜０．０５），弱级（１～２级）表达的患者预后好。结论ＰＣＮＡ检测可作为判断胃癌生物学行为和预后的一项良好指标。
Objective To study the expression and clinical significance of proliferating cell nuclear antigen (PCNA) in gastric benign and malignant tumors. Methods Using immunohistochemical technique (ABC method), the semi-quantitative detection of PCNA in gastric cancer tissue sections (10 gastric adenomatous polyps and 94 gastric cancers) was performed to observe its relationship with clinicopathological parameters and prognosis of gastric cancer. Results (1) There were significant differences in PCNA index between different gastric mucosal pathological conditions (P value between each group was less than 0.01); (2) PCNA index had no correlation with patient’s gender and age (P>0.05). The tumor growth pattern, tissue type, serosal layer invasion or not, lymph node metastasis and clinical stage were closely related (P values were less than 0.05); (3) PCNA index was closely related to the prognosis of gastric cancer (P < 0 .05). Weak (grades 1 to 2) patients have a good prognosis. Conclusion The detection of PCNA can be used as a good indicator to judge the biological behavior and prognosis of gastric cancer.