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[目的 ]通过实验室细胞染毒模型来揭示无铅汽油的使用能否减低汽车尾气颗粒有机提取物的细胞膜毒性。 [方法 ]在发动机台架试验上 ,分别使用市售 90 #含铅及无铅两种汽油来制造尾气。收集尾气的颗粒物及其吸附有机物 ,并检测尾气中HC与CO浓度。将体外培养中国仓鼠肺细胞 (CHL)暴露于尾气的提取物中 ,通过检测细胞培养液中乳酸脱氢酶(LDH)及细胞内K+ 及Ca2 + 浓度来分析细胞内外物质交换的变化 ,从而说明不同汽油排出尾气颗粒有机提取物的细胞膜毒性。 [结果 ]随着对细胞染毒剂量的增加 ,培养液中的LDH浓度以及细胞内的Ca2 + 浓度有增加的趋势 ,而细胞内K+ 浓度有降低的趋势 ,这种变化呈剂量 反应关系。统计学检验表明 ,无铅汽油尾气颗粒提取物的细胞膜毒性与对照组及其他实验组有显著性的差别。 [结论 ]无铅汽油的使用能减少尾气中CO、HC及颗粒物的排放。与含铅汽油相比较 ,无铅汽油的颗粒吸附有机物的细胞膜毒性有所降低
[Objective] To reveal whether the use of unleaded gasoline can reduce the cytotoxicity of organic extracts of vehicle exhaust particles by laboratory cell model. [Method] On the engine bench test, tail gas was manufactured by using 90 # lead and lead-free gasoline respectively. Collect exhaust particulate matter and its adsorption of organic matter, and detect exhaust gas HC and CO concentration. The in vitro culture of Chinese hamster lung cells (CHL) was exposed to the extract of the tail gas, and the changes of intracellular and extracellular material exchanges were analyzed by measuring the concentration of lactate dehydrogenase (LDH) and K + and Ca2 + in the cell culture medium Cell Membrane Toxicity of Organic Extracts from Different Gas Exhausted Exhausted Particles. [Result] With the increase of the dose of the cells, the concentration of LDH in the culture medium and the intracellular Ca2 + concentration tended to increase while the concentration of K + in the cells tended to decrease. The change was dose - dependent. Statistical tests showed that the cytotoxicity of unleaded gasoline tail gas extract was significantly different from the control group and other experimental groups. [Conclusion] The use of unleaded gasoline can reduce the emission of CO, HC and particulate matter in the exhaust gas. Compared with leaded gasoline, unleaded gasoline particles adsorbed organic matter decreased the toxicity of the cell membrane