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目的建立脂降宁片中葛根的含量测定方法。方法采用HPLC法,以乙腈-水(11:89)为流动相,迪马-platisil ODS C18(5μm,250×4.6mm)色谱柱,流速1.0ml/min,柱温30℃,进样量10μl,检测波长250nm。结果葛根素在0.02582μg~0.7746μg的范围内线性关系良好,r=0.9998,加样回收率为99.7%,RSD=1.1%(n=6),阴性样品无干扰。3批样品含量分别为1.93mg/g、0.584mg/g、0.411mg/g。结论此方法专属性强,准确,简便,可作为脂降宁片中葛根含量的测定方法。
Objective To establish a method for the determination of Pueraria lobata in Zhijinning tablets. Methods The HPLC method was developed on the mobile phase of acetonitrile-water (11:89) with a mobile phase of Dima-Platisil ODS C18 (5μm, 250 × 4.6mm) at a flow rate of 1.0ml / , Detection wavelength 250nm. Results Puerarin showed good linearity in the range of 0.02582μg ~ 0.7746μg with r = 0.9998. The recovery was 99.7% with RSD of 1.1% (n = 6). The negative samples showed no interference. Three batches of samples were 1.93mg / g, 0.584mg / g, 0.411mg / g respectively. Conclusion This method is specific, accurate and simple, and can be used as a method for determination of Pueraria lobata in Zhijinning tablets.