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通过对紫叶风箱果组培外植体选择、灭菌、启动分化、初代、继代、生根培养基进行对比筛选研究,以期建立紫叶风箱果的再生体系。结果表明:外植体以春季幼嫩茎尖为宜,取材的最适季节3-5月(温室环境),消毒灭菌的最适方法为0.1%HgCl_2与2%NaClO1:1混合液体浸泡8 min;最适的诱导培养基为改良MS+6-BA2 mg/L+GA_30.5 mg/L+IBA0.05 mg/L,增殖培养基为改良MS+6-BA2 mg/L+GA_30.5 mg/L,生根培养基为1/2MS+IBA1.5 mg/L+NAA3.0 mg/L+IAA0.5 mg/L。
By selecting, sterilizing, initiating differentiation, primary passage, secondary passage and rooting medium of the purple leaf bellows tissue culture explants, the comparative screening study was conducted in order to establish the regenerative system of purple leaf bellows. The results showed that the best explants were the young shoot tips in spring and the optimum season for harvesting in the greenhouse from March to May (greenhouse environment). The best method of disinfection and sterilization was that the mixed solution of 0.1% HgCl 2 and 2% NaClO 1: 1 soaked 8 min. The best induction medium was MS + 6-BA2 mg / L + GA_30.5 mg / L + IBA 0.05 mg / L, and the proliferation medium was modified MS + 6- BA2 mg / L + GA_30.5 The rooting medium was 1 / 2MS + IBA 1.5 mg / L + NAA 3.0 mg / L + IAA 0.5 mg / L.